Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

Nurliyana Mohamad; Fauzul Aidil Mohd Roseli; Mohammad Noor Amal Azmai; Mohd Zamri Saad; Ina Salwany Md Yasin; Nor Amalina Zulkiply; Nurrul Shaqinah Nasruddin

doi:10.1002/aah.10055

journal article Jan 28, 2019

Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

Nurliyana Mohamad Fauzul Aidil Mohd Roseli Mohammad Noor Amal Azmai

Mohd Zamri Saad Ina Salwany Md Yasin Nor Amalina Zulkiply Nurrul Shaqinah Nasruddin

Journal of Aquatic Animal Health Vol. 31 No. 1 pp. 88-96 · Oxford University Press (OUP)

View at Publisher Save 10.1002/aah.10055

Abstract

Abstract
In September 2016, a marine fish farm operator in Selangor, Malaysia, reported a disease outbreak affecting juvenile hybrid groupers (Camouflage Grouper Epinephelus polyphekadion × Tiger Grouper E. fuscoguttatus). The average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 d. The affected hybrid groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. Microscopically, general congestion of the brains and internal organs was evident. Vibrio harveyi and V. alginolyticus were successfully isolated from the diseased fish. The isolated pathogens were found to be sensitive to oxytetracycline and tetracycline, but resistant towards ampicillin and vancomycin. Experimental infections using the isolated V. harveyi (108 CFU/mL), V. alginolyticus (108 CFU/mL), and concurrent infection by V. harveyi (108 CFU/mL) and V. alginolyticus (108 CFU/mL) in juvenile Asian Seabass Lates calcarifer resulted in 60, 100, and 100% mortality, respectively, within 240 h postinfection. The experimentally infected Asian Seabass demonstrated similar clinical signs and histopathological changes as the naturally infected hybrid groupers. However, concurrently infected fish demonstrated severe clinical signs and histopathological changes compared with single infections. These results suggest that both isolates of Vibrio are pathogenic to fish and responsible for the disease outbreak. However, concurrent infection involving V. alginolyticus and V. harveyi leads to a more devastating impact to the cultured fish. This is the first report of concurrent Vibrio infection in cultured marine fish in Malaysia.

Topics

No keywords indexed for this article. Browse by subject →

References

48

[1]

Abdullah "The presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage cultured fish: role of fish size, water physicochemical parameters and relationships among the pathogens" Aquaculture Reports (2017) 10.1016/j.aqrep.2017.06.001

[2]

Aisyhah "Streptococcus agalactiae isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics" Journal of Fish Diseases (2015) 10.1111/jfd.12351

[3]

Akaayli "Characterization of Vibrio alginolyticus isolates from diseased culture Gilthead Sea Bream, Sparus aurata" Israeli Journal of Aquaculture–Bamidgeh (2008) 10.46989/001c.20487

[4]

Amal "A case of natural co-infection of Tilapia Lake Virus and Aeromonas veronii in a Malaysian red hybrid tilapia (Oreochromis niloticus × O. mossambicus) farm experiencing high mortality" Aquaculture (2018) 10.1016/j.aquaculture.2017.11.019

[5]

Amal "The effects of fish gender on susceptibility to acute Streptococcus agalactiae infection in Javanese Medaka Oryzias javanicus" Microbial Pathogenesis (2018) 10.1016/j.micpath.2017.11.069

[6]

Arif "Bacteria associated with mass mortality of hybrid grouper Epinephelus sp. in East Java Province, Indonesia" International Journal of Fisheries and Aquatic Studies (2016)

[7]

Aslizah (2018)

[8]

Assis "Natural coinfection by Streptococcus agalactiae and Francisella noatunensis subsp. orientalis in farmed Nile Tilapia (Oreochromis niloticus L.)" Journal of Fish Diseases (2016) 10.1111/jfd.12493

[9]

Austin (2012) 10.1007/978-94-007-4884-2

[10]

Austin "Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates" Letters in Applied Microbiology (2006) 10.1111/j.1472-765x.2006.01989.x

[11]

Avendaño-Herrera "Tenacibaculosis infection in marine fish caused by Tenacibaculum maritimum: a review" Diseases of Aquatic Organisms (2006) 10.3354/dao071255

[12]

Bowden "Comparative pathogenicity of Vibrio spp., Photobacterium damselae spp. damselae and five isolates of Aeromonas salmonicida ssp. achromogenes in juvenile Atlantic Halibut (Hippoglossus hippoglossus)" Journal of Fish Diseases (2018) 10.1111/jfd.12679

[13]

Bunlipatanon "Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus)" Aquaculture Research (2017) 10.1111/are.13008

[14]

Cabello "Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health" Environmental Microbiology (2013) 10.1111/1462-2920.12134

[15]

Chimetto "Genomic diversity of Vibrios associated with the Brazilian coral Mussismilia hispida and its sympatric zoanthids (Palythoa caribaerorum, Palythoa variabilis, and Zoanthus solanderi)" Journal of Applied Microbiology (2009) 10.1111/j.1365-2672.2009.04149.x

[16]

CLSI (Clinical and Laboratory Standards Institute) (2011)

[17]

Crumlish "Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila" Journal of Fish Diseases (2010) 10.1111/j.1365-2761.2010.01173.x

[18]

Dong "Concurrent infections of Flavobacterium columnare and Edwardsiella ictaluri in Striped Catfish, Pangasianodon hypophthalmus, in Thailand" Aquaculture (2015) 10.1016/j.aquaculture.2015.05.046

[19]

Recovery of Vibrio harveyi from scale drop and muscle necrosis disease in farmed barramundi, Lates calcarifer in Vietnam

H T Dong, S Taengphu, P Sangsuriya et al.

Aquaculture 2017 10.1016/j.aquaculture.2017.02.005

[20]

FAO (Food and Agriculture Organization of the United Nations) (2017)

[21]

Haenen "Vibriosis in aquaculture. 16th EAFP Conference, Tampere, Finland, September 4, 2013" Bulletin of the European Association of Fish Pathologists (2014)

[22]

Harikrishnan "Molecular studies, disease status and prophylactic measures in grouper aquaculture: economic importance, diseases and immunology" Aquaculture (2010) 10.1016/j.aquaculture.2010.09.011

[23]

Hashem "Vibrio harveyi infection in Arabian surgeon fish (Acanthurus sohal) of Red Sea at Hurghada, Egypt" Egyptian Journal of Aquatic Research (2013) 10.1016/j.ejar.2013.10.006

[24]

Karlsen "Co-infection of Atlantic Salmon (Salmo salar), by Moritella viscosa and Aliivibrio wodanis, development of disease and host colonization" Veterinary Microbiology (2014) 10.1016/j.vetmic.2014.03.011

[25]

Kiriyakit "Successful hybridization of groupers (Epinephelus coioides × Epinephelus lanceolatus) using cryopreserved sperm" Aquaculture (2011) 10.1016/j.aquaculture.2011.05.012

[26]

Korun "Antibiotic resistance and plasmid profile of Vibrio alginolyticus strains isolated from cultured European Sea Bass (Dicentrarchus labrax, L.)" Bulletin of the Veterinary Institute in Pulawy (2013) 10.2478/bvip-2013-0032

[27]

Kotob "The impact of co-infections on fish: a review" Veterinary Research (2016) 10.1186/s13567-016-0383-4

[28]

Krupesha-Sharma "First report of isolation and characterization of Photobacterium damselae subsp. damselae from cage-farmed Cobia (Rachycentron canadum)" Journal of Fish Diseases (2016) 10.1111/jfd.12557

[29]

Krupesha-Sharma "Vibrio alginolyticus infection in Asian Seabass (Lates calcarifer, Bloch) reared in open sea floating cages in India" Aquaculture Research (2012) 10.1111/j.1365-2109.2011.03013.x

[30]

Kumar (2015)

[31]

Liu "Investigation of Vibrio alginolyticus, V. harveyi, and V. parahaemolyticus in large Yellow Croaker, Pseudosciaena crocea (Richardson), reared in Xiangshan Bay, China" Aquaculture Reports (2016) 10.1016/j.aqrep.2016.04.004

[32]

Liu "Isolation and characterization of pathogenic Vibrio harveyi (V. carchariae) from the farmed marine Cobia fish Rachycentron canadum L. with gastroenteritis syndrome" World Journal of Microbiology and Biotechnology (2004) 10.1023/b:wibi.0000040402.44340.0e

[33]

Manilal "Virulence of vibrios isolated from diseased black tiger shrimp, Penaeus monodon, Fabricius" Journal of the World Aquaculture Society (2010) 10.1111/j.1749-7345.2010.00375.x

[34]

Mohi "Histopathological studies on Vibrio harveyi infected Tiger Puffer, Takifugu rubripes (Temminck et Schlegel), cultured in Japan" Journal of Fish Diseases (2010) 10.1111/j.1365-2761.2010.01184.x

[35]

Pedersen "Photobacterium damselae subsp. damselae, an emerging pathogen in Danish Rainbow Trout, Oncorhynchus mykiss (Walbaum), mariculture" Journal of Fish Diseases (2009) 10.1111/j.1365-2761.2009.01041.x

[36]

Qin "Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara" Acta Oceanologica Sinica (2006)

[37]

Rameshkumar "Microbiological and histopathological investigations of Vibrio alginolyticus infection in Cobia Rachycentron canadum (Linnaeus, 1766) cultured in sea cage" Indian Journal of Fisheries (2014)

[38]

Rameshkumar "Isolation and characterization of pathogenic Vibrio alginolyticus from sea cage cultured Cobia (Rachycentron canadum (Linnaeus 1766)) in India" Letters in Applied Microbiology (2017) 10.1111/lam.12800

[39]

Ransangan "Characterization and experimental infection of Vibrio harveyi isolated from diseased Asian Seabass (Lates calcarifer)" Malaysian Journal of Microbiology (2012)

[40]

Ransangan "Identification of Vibrio harveyi isolated from diseased Asian Seabass (Lates calcarifer) by use of 16S ribosomal DNA sequencing" Journal of Aquatic Animal Health (2009) 10.1577/h09-002.1

[41]

Sarjito "Phylogenetic diversity of the causative agents of vibriosis associated with groupers fish from Karimunjawa Islands, Indonesia" Current Research in Bacteriology (2009)

[42]

Shen "Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂" Journal of Fish Diseases (2017) 10.1111/jfd.12609

[43]

Sun "Comparative transcriptomic study of muscle provides new insights into the growth superiority of a novel grouper hybrid" PLoS ONE (2016) 10.1371/journal.pone.0168802

[44]

Tendencia "Vibrio harveyi isolated from cage-cultured Seabass Lates calcarifer Bloch in the Philippines" Aquaculture Research (2002) 10.1046/j.1365-2109.2002.00688.x

[45]

Won "Pathogenicity of Vibrio harveyi to cultured marine fishes in Korea" Aquaculture (2008) 10.1016/j.aquaculture.2008.08.013

[46]

Zhang "Outbreak of a novel disease associated with Vibrio mimicus infection in fresh water cultured Yellow Catfish, Pelteobagrus fulvidraco" Aquaculture (2014) 10.1016/j.aquaculture.2014.04.039

[47]

Zhao "Characterization of two phenotypes of Photobacterium damselae subsp. damselae isolated from diseased juvenile Trachinotus ovatus reared in cage mariculture" Journal of the World Aquaculture Society (2009) 10.1111/j.1749-7345.2009.00251.x

[48]

Zhu "The high prevalence of pathogenic Vibrio harveyi with multiple antibiotic resistance in scale drop and muscle necrosis disease of the hybrid grouper, Epinephelus fuscoguttatus (♀) × E. lanceolatus (♂), in China" Journal of Fish Diseases (2017)

Cited By

75

Rapid and Portable Gold Nanoparticle-Based Nanosensors for Vibrio spp. Detection from Aquaculture Water and Fish Tissues

Khin Moh Moh Aung, Laura Sutarlie · 2026

ACS Applied Nano Materials

Transcriptome Analysis of Host Anti-Vibrio harveyi Infection Revealed the Pathogenicity of V. harveyi to American Eel (Anguilla rostrata)

Minxia Chen, Qijuan Wan · 2024

Marine Biotechnology

Pathology and pathogenesis of Vibrio infection in fish: A review

Tilusha Manchanayake, Annas Salleh · 2023

Aquaculture Reports

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Xinxin He, Brian Austin · 2020

Marine Life Science & Technolog...

Metrics

75

Citations

48

References

Details

Published: Jan 28, 2019
Vol/Issue: 31(1)
Pages: 88-96
License: View

Authors

N

Nurliyana Mohamad

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

F

Fauzul Aidil Mohd Roseli

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

M

Mohammad Noor Amal Azmai

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; and Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

M

Mohd Zamri Saad

Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; and Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

I

Ina Salwany Md Yasin

Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; and Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

N

Nor Amalina Zulkiply

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

N

Nurrul Shaqinah Nasruddin

Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia

Funding

Ministry of Higher Education Award: 6369100

Universiti Putra Malaysia Award: GP-IPB/2016/9484101

Cite This Article

Nurliyana Mohamad, Fauzul Aidil Mohd Roseli, Mohammad Noor Amal Azmai, et al. (2019). Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia. Journal of Aquatic Animal Health, 31(1), 88-96. https://doi.org/10.1002/aah.10055

Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

You May Also Like