An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections
Wound infections pose a significant challenge in healthcare, and traditional antibiotic treatments often result in the development of resistant pathogens. Addressing this gap, ProGel is introduced, a living hydrogel created by entrapping probiotic
Lactobacillus plantarum
as a therapeutic component within a gelatin matrix. With a double‐syringe system, ProGel can be easily mixed and applied, conforming swiftly to any wound shape and forming hydrogel in situ. It also demonstrates robust mechanical and self‐healing properties owing to the Schiff‐base bonds. ProGel sustains more than 80% viability of the entrapped
L. plantarum
while restricting their escape from the hydrogel. After a week of storage, more than 70% viability of the entrapped
L. plantarum
is preserved. Importantly, ProGel exhibits broad‐spectrum antimicrobial efficacy against pathogens commonly associated with wound infections, i.e.,
Pseudomonas aeruginosa
(7Log reduction),
Staphylococcus aureus
(3‐7Log reduction), and
Candida albicans
(40–70% reduction). Moreover, its cytocompatibility is affirmed through coculture with human dermal fibroblasts. The effectiveness of ProGel is further highlighted in more clinically relevant tests on human skin wound models infected with
P. aeruginosa
and
S. aureus
, where it successfully prevents the biofilm formation of these pathogens. This study showcases an injectable living hydrogel system for the management of complex wound infections.
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- Published
- Jul 14, 2024
- Vol/Issue
- 13(27)
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