A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria

Arturo Jiménez‐Sánchez; Eric K. Lei; Shana O. Kelley

doi:10.1002/anie.201802796

journal article Jun 19, 2018

A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria

Arturo Jiménez‐Sánchez Eric K. Lei Shana O. Kelley

Angewandte Chemie International Edition Vol. 57 No. 29 pp. 8891-8895 · Wiley

View at Publisher Save 10.1002/anie.201802796

Abstract

AbstractThe measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle‐specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity (ηm) and micropolarity (ϵm), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of ηm and ϵm by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of ηm and ϵm in the presence of a variety of agents perturbing cellular function, and the observed changes can also be correlated with alterations in mitochondrial network morphology and motility. This strategy represents a promising tool for the analysis of subtle changes in organellar structure.

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Metrics

185

Citations

38

References

Details

Published: Jun 19, 2018
Vol/Issue: 57(29)
Pages: 8891-8895
License: View

Authors

A

Arturo Jiménez‐Sánchez

Department of Biochemistry Faculty of Medicine University of Toronto Toronto Ontario M5S 1A8 Canada

E

Eric K. Lei

Department of Biochemistry Faculty of Medicine University of Toronto Toronto Ontario M5S 1A8 Canada

S

Shana O. Kelley

Department of Biochemistry Faculty of Medicine University of Toronto Toronto Ontario M5S 1A8 Canada; Department of Pharmaceutical Sciences Leslie Dan Faculty of Pharmacy Institute for Biomedical and Biomaterials Engineering Departments of Biochemistry and Chemistry University of Toronto Toronto Ontario M5S 1A8 Canada

Funding

National Institutes of Health Award: R01GM116886

Canadian Institutes of Health Research

Cite This Article

Arturo Jiménez‐Sánchez, Eric K. Lei, Shana O. Kelley (2018). A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria. Angewandte Chemie International Edition, 57(29), 8891-8895. https://doi.org/10.1002/anie.201802796

A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria

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