Surface modification of poly(styrene) by the attachment of an antimicrobial peptide

P. Appendini; J. H. Hotchkiss

doi:10.1002/app.1476

journal article May 03, 2001

Surface modification of poly(styrene) by the attachment of an antimicrobial peptide

P. Appendini J. H. Hotchkiss

Journal of Applied Polymer Science Vol. 81 No. 3 pp. 609-616 · Wiley

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Abstract

AbstractPolymers that directly inhibit the growth of microorganisms at their surface are potentially useful. To investigate the feasibility of such materials, poly(styrene) (PS) resin beads that had had poly(ethyleneglycol) (PEG) grafted onto the surface were further derivatized by covalently linking an antimicrobial peptide onto the surface. The antimicrobial peptide was composed of 8 lysine and 7 leucine (6K8L) residues. The resulting surface‐modified polystyrene (SMPS) was microcidal in a concentration and time‐dependent manner against several micro‐organisms includingE. coliO157 : H7,L. monocytogenes, S. aureus, P. fluorescens,andK. marxianuswhen suspended in phosphate buffer. The SMPS inhibited the growth of pathogenicE. coliO157 : H7 in trypticase soy broth. SMPS was bactericidal at pH 3.5 to 7, retained activity after heating to 200°C for 30 min, and could be extensively washed without loss of antimicrobial activity. Bioassays and HPLC analyses of buffer that had been preincubated with SMPS indicated that antimicrobial activity may have been due, at least in part, to the slow release of a peptide–PEG ligand from the PS to the buffer. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 609–616, 2001

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Details

Published: May 03, 2001
Vol/Issue: 81(3)
Pages: 609-616
License: View

Authors

Cite This Article

P. Appendini, J. H. Hotchkiss (2001). Surface modification of poly(styrene) by the attachment of an antimicrobial peptide. Journal of Applied Polymer Science, 81(3), 609-616. https://doi.org/10.1002/app.1476

Surface modification of poly(styrene) by the attachment of an antimicrobial peptide

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