Immobilization of glucose oxidase on nonwoven fabrics with bombyx mori silk fibroin gel

Tetsuo Asakura; Motohiro Kitaguchi; Makoto Demura; Harutoshi Sakai; Keiichi Komatsu

doi:10.1002/app.1992.070460106

journal article Sep 05, 1992

Immobilization of glucose oxidase on nonwoven fabrics with bombyx mori silk fibroin gel

Tetsuo Asakura

Motohiro Kitaguchi Makoto Demura Harutoshi Sakai Keiichi Komatsu

Journal of Applied Polymer Science Vol. 46 No. 1 pp. 49-53 · Wiley

View at Publisher Save 10.1002/app.1992.070460106

Abstract

AbstractThe enzyme glucose oxidase (GOD) was immobilized on the nonwoven fabrics, which have excellent properties in diffusivity of substrates, mechanical strength, and handling, with Bombyx mori silk fibroin gel. The nonwoven fabrics of silk fibroin, viscose rayon, poly‐ethyleneterephthalate, 6‐nylon, and polypropylene with activated surface by fluoline treatment were used. The stabilities of GOD to heat or pH changes were much improved by the immobilization as well as the case of the GOD immobilized in the silk fibroin membrane. Among nonwoven fabrics, silk fibroin was the most excellent support material for the immobilization of GOD although all nonwoven fabrics used here are able to be used as the support materials. The increase of the sensitivity was observed when the glucose sensor was made with the GOD immobilized on nonwoven silk fabrics as four times compared with the case of the GOD immobilized in the silk fibroin membrane.

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Cited By

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Immobilization of glucose oxidase in the composite membrane of regenerated silk fibroin and poly(vinyl alcohol): application to an amperometric glucose sensor

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Metrics

38

Citations

18

References

Details

Published: Sep 05, 1992
Vol/Issue: 46(1)
Pages: 49-53
License: View

Authors

T

Tetsuo Asakura

Department of Biotechnology, Tokyo University of Agriculture and Technology - Kaganei Campus, Koganei, Japan

Cite This Article

Tetsuo Asakura, Motohiro Kitaguchi, Makoto Demura, et al. (1992). Immobilization of glucose oxidase on nonwoven fabrics with bombyx mori silk fibroin gel. Journal of Applied Polymer Science, 46(1), 49-53. https://doi.org/10.1002/app.1992.070460106

Immobilization of glucose oxidase on nonwoven fabrics with bombyx mori silk fibroin gel

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