Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR

Galo Cárdenas; Gustavo Cabrera; Edelio Taboada; S. Patricia Miranda

doi:10.1002/app.20647

journal article Jun 11, 2004

Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR

Galo Cárdenas Gustavo Cabrera Edelio Taboada S. Patricia Miranda

Journal of Applied Polymer Science Vol. 93 No. 4 pp. 1876-1885 · Wiley

View at Publisher Save 10.1002/app.20647

Abstract

AbstractThe full characterization of chitin obtained from squid, shrimp, prawn, lobsters, and king crab is reported. Elemental analysis, including metals such as Ca, Mg, Zn, Cd, Hg, Cr, Mn, Cu, and Pb, was performed, which is quite relevant because the skeleton composition is slightly different for each species. The morphology was studied by means of TEM and their compositions were determined by energy‐dispersive X‐ray analysis. 13C cross polarization/magic angle spinning NMR was applied to determine the chemical shift of all the carbons and the difference between them. Chitin was isolated by using chemical methods, alternating hydrochloric acid and sodium hydroxide. The α‐chitin from shrimp, prawn, lobsters, and king crabs showed two signals at 73.7 and 75.6 ppm. Meanwhile, the β‐chitin from squid exhibited one signal at 75.2 ppm. FTIR studies were used to analyze α‐chitin from shrimp and β‐chitin from squid. The α‐chitin exhibited amide I vibration modes at 1660 and 1627 cm−1, whereas the β‐chitin showed one band at 1656 cm−1. X‐ray diffraction showed that α‐chitin is orthorhombic (a = 4.74 Å, b = 18.86 Å, and c = 10.32 Å) and β‐chitin had a monoclinic dihydrated form (a = 4.80 Å, b = 10.40 Å, c = 11.10 Å, and β = 97°). © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 1876–1885, 2004

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Details

Published: Jun 11, 2004
Vol/Issue: 93(4)
Pages: 1876-1885
License: View

Authors

Funding

Innova Bío-Bío Award: FIT B1-050

Cite This Article

Galo Cárdenas, Gustavo Cabrera, Edelio Taboada, et al. (2004). Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR. Journal of Applied Polymer Science, 93(4), 1876-1885. https://doi.org/10.1002/app.20647

Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/mass angle spinning NMR

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