Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa

Timothy J. Pegg; Daniel K. Gladish; Robert L. Baker

doi:10.1002/aps3.11437

journal article Open Access Jun 01, 2021

Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa

Timothy J. Pegg

Daniel K. Gladish

Robert L. Baker

Applications in Plant Sciences Vol. 9 No. 6 · Wiley

View at Publisher Save 10.1002/aps3.11437

Abstract

PremiseFluorescence microscopy is an effective tool for viewing plant internal anatomy. However, using fluorescent antibodies or labels hinders throughput. We present a minimal protocol that takes advantage of inherent autofluorescence and aldehyde‐induced fluorescence in plant cellular and subcellular structures to markedly increase throughput in cellular and ultrastructural visualization.Methods and ResultsTwelve species distributed across the plant phylogeny were each subjected to five fixative treatments: 1% paraformaldehyde and 2% glutaraldehyde, 2% paraformaldehyde, 2% glutaraldehyde, formalin‐acid‐alcohol (FAA), and 70% ethanol. Samples were prepared by embedding and mechanically sectioning or via whole mount. A confocal laser scanning system was used to collect micrographs. We evaluated and compared fixative influence on sample structural preservation and tissue autofluorescence.ConclusionsFormaldehyde fixation of Viridiplantae taxa samples generates useful structural data while requiring no additional histological staining or clearing. In addition, a fluorescence‐capable microscope is the only specialized equipment required for image acquisition. The minimal protocol developed in this experiment enables high‐throughput sample processing by eliminating the need for multi‐day preparations.

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Details

Published: Jun 01, 2021
Vol/Issue: 9(6)
License: View

Authors

T

Timothy J. Pegg

Department of Biology Miami University Oxford Ohio 45056 USA; Graduate Program in Botany Miami University Oxford Ohio 45056 USA

D

Daniel K. Gladish

Department of Biology Miami University Oxford Ohio 45056 USA; Graduate Program in Botany Miami University Oxford Ohio 45056 USA

R

Robert L. Baker

Department of Biology Miami University Oxford Ohio 45056 USA; Graduate Program in Botany Miami University Oxford Ohio 45056 USA

Cite This Article

Timothy J. Pegg, Daniel K. Gladish, Robert L. Baker (2021). Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa. Applications in Plant Sciences, 9(6). https://doi.org/10.1002/aps3.11437

Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa

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