Spatially resolved detection of small molecules from press‐dried plant tissue using MALDI imaging

Abstract

Premise
Matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) is a chemical imaging method that can visualize spatial distributions of particular molecules. Plant tissue imaging has so far mostly used cryosectioning, which can be impractical for the preparation of large‐area imaging samples, such as full flower petals. Imaging unsectioned plant tissue presents its own difficulties in extracting metabolites to the surface due to the waxy cuticle.

Methods
We address this by using established delipidation techniques combined with a solvent vapor extraction prior to applying the matrix with many low‐concentration sprays.

Results
Using this procedure, we imaged tissue from three different plant species (two flowers and one carnivorous plant leaf). Material factorization analysis of the resulting data reveals a wide range of plant‐specific small molecules with varying degrees of localization to specific portions of the tissue samples, while facilitating detection and removal of signal from background sources.

Conclusions
This work demonstrates applicability of MALDI‐MSI to press‐dried plant samples without freezing or cryosectioning, setting the stage for spatially resolved molecule identification. Increased mass resolution and inclusion of tandem mass spectrometry are necessary next steps to allow more specific and reliable compound identification.

Topics

No keywords indexed for this article. Browse by subject →

References

51

[1]

Adler D. andS. T.Kelly.2020. vioplot: violin plot. R package version 0.3.7. Website:https://github.com/TomKellyGenetics/vioplot[accessed 13 July 2023].

[2]

Alston R. E. (1955)

[3]

10.1104/pp.16.00055

[4]

10.1016/j.biotechadv.2015.08.001

[5]

10.1201/b17040

[6]

10.1162/jmlr.2003.3.4-5.993

Applied Physics Letters 10.1162/jmlr.2003.3.4-5.993

[7]

10.1016/j.patcog.2007.09.010

[8]

10.1002/prot.25095

[9]

10.1111/j.1365-313x.2008.03507.x

[10]

10.1038/nmeth1094

[11]

10.1039/c9ay00226j

[12]

10.1007/s13361-016-1422-0

[13]

10.18637/jss.v040.i08

[14]

10.1016/1044-0305(96)00027-x

[15]

10.1002/cbdv.201200359

[16]

10.1146/annurev-arplant-050718-095910

[17]

10.1016/j.plantsci.2003.08.001

[18]

Ferreyra M. L. F. "Flavonoids: Biosynthesis, biological functions, and biotechnological applications" Frontiers in Plant Science (2012)

[19]

Garnier S. N.Ross R.Rudis A. P.Camargo M.Sciaini andC.Scherer.2021. viridis: Colorblind‐friendly color maps for R. R package version 0.6.2. Website:https://sjmgarnier.github.io/viridis/[accessed 13 July 2023].

[20]

10.1137/1.9781611976410

[21]

10.3390/plants10020186

[22]

10.3390/molecules27154811

[23]

10.1093/aob/mcz191

[24]

10.1021/jf801297w

[25]

Plant Secondary Metabolite Diversity and Species Interactions

André Kessler, Aino Kalske

Annual Review of Ecology, Evolution, and Systemati... 10.1146/annurev-ecolsys-110617-062406

[26]

10.1111/j.1365-313x.2005.02508.x

[27]

10.1038/s41598-019-38706-4

[28]

10.2307/2281082

[29]

Lee D. D. "Algorithms for non‐negative matrix factorization" Advances in Neural Information Processing Systems (2001)

[30]

10.1002/aps3.11546

[31]

10.1084/jem.20191920

[32]

10.1080/07388551.2020.1731414

[33]

10.1111/nph.12526

[34]

10.1021/ac201123t

[35]

10.1016/j.tplants.2004.07.006

[36]

10.1111/j.1469-8137.2011.03970.x

[37]

Qiu Y. andJ.Mei.2022. RSpectra: Solvers for large‐scale eigenvalue and SVD problems. R package version 0.16‐1. Website:https://CRAN.R-project.org/package=RSpectra[accessed 13 July 2023].

[38]

R Core Team.2022. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna Austria. Website:https://www.R-project.org/[accessed 13 July 2023].

[39]

10.1093/aob/mcaa038

[40]

10.1016/b978-0-12-816460-0.00004-6

[41]

10.1073/pnas.2114420119

[42]

10.1111/pce.13653

[43]

Slack A. (1979)

[44]

10.1111/nph.13560

[45]

10.1021/acs.analchem.8b03815

[46]

Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids

Yoshikazu Tanaka, Nobuhiro Sasaki, Akemi Ohmiya

The Plant Journal 10.1111/j.1365-313x.2008.03447.x

[47]

10.1016/s1359-6446(97)01167-7

[48]

10.1021/acs.analchem.8b05112

[49]

10.1021/ac200998a

[50]

10.1039/d0an02118k

Showing 50 of 51 references

Metrics

2

Citations

51

References

Details

Published: Sep 01, 2023
Vol/Issue: 11(5)
License: View

Authors

Z

Zane G. Long

Department of Chemistry University of California Irvine California 92697‐2025 USA

J

Jonathan V. Le

Department of Molecular Biology and Biochemistry University of California Irvine California 92697‐3900 USA

B

Benjamin B. Katz

Department of Chemistry University of California Irvine California 92697‐2025 USA

B

Belen G. Lopez

Department of Chemistry University of California Irvine California 92697‐2025 USA

E

Emily D. Tenenbaum

Piscataway High School Piscataway New Jersey 08854 USA

B

Bonnie Semmling

The Chrysler Herbarium and Mycological Collection, School of Environmental and Biological Sciences Rutgers University New Brunswick New Jersey 08901 USA

R

Ryan J. Schmidt

Department of Ecology, Evolution, and Natural Resources Rutgers University New Brunswick New Jersey 08901 USA

F

Felix Grün

Department of Chemistry University of California Irvine California 92697‐2025 USA

C

Carter T. Butts

Departments of Sociology, Statistics, Computer Science, and Electrical Engineering and Computer Science University of California Irvine California 92697 USA

R

Rachel W. Martin

Department of Chemistry University of California Irvine California 92697‐2025 USA; Department of Molecular Biology and Biochemistry University of California Irvine California 92697‐3900 USA

Cite This Article

Zane G. Long, Jonathan V. Le, Benjamin B. Katz, et al. (2023). Spatially resolved detection of small molecules from press‐dried plant tissue using MALDI imaging. Applications in Plant Sciences, 11(5). https://doi.org/10.1002/aps3.11539

Spatially resolved detection of small molecules from press‐dried plant tissue using MALDI imaging

You May Also Like