EVALUATION OF IN VITRO AND IN VIVO EFFECTS OF SEMIPURIFIED PROTEINASE INHIBITORS FROM THEOBROMA SEEDS ON MIDGUT PROTEASE ACTIVITY OF LEPIDOPTERAN PEST INSECTS

Luis Cesar Maffei Sartini Paulillo; Alexandre Magno Sebbenn; Maria Tereza Vitral de Carvalho Derbyshire; Aristóteles Góes‐Neto; Marco Aurélio de Paula Brotto; Antonio Figueira

doi:10.1002/arch.21038

journal article Jul 16, 2012

EVALUATION OF IN VITRO AND IN VIVO EFFECTS OF SEMIPURIFIED PROTEINASE INHIBITORS FROM THEOBROMA SEEDS ON MIDGUT PROTEASE ACTIVITY OF LEPIDOPTERAN PEST INSECTS

Luis Cesar Maffei Sartini Paulillo Alexandre Magno Sebbenn Maria Tereza Vitral de Carvalho Derbyshire Aristóteles Góes‐Neto

Marco Aurélio de Paula Brotto Antonio Figueira

Archives of Insect Biochemistry and Physiology Vol. 81 No. 1 pp. 34-52 · Wiley

View at Publisher Save 10.1002/arch.21038

Abstract

We have characterized in vitro and in vivo effects of trypsin inhibitors from Theobroma seeds on the activity of trypsin‐ and chymotrypsin‐like proteins from Lepidopteran pest insects. The action of semipurified trypsin inhibitors from Theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of N‐Benzoyl‐DL‐Arginine‐p‐Nitroanilide (BAPA) and N‐Succinyl‐Ala‐Ala‐Pho‐Phe p‐Nitroanilide (S‐(Ala)2ProPhe‐pNA). Proteinase inhibitor activities from Theobroma cacao and T. obovatum seeds were the most effective in inhibiting trypsin‐like proteins, whereas those from T. obovatum and T. sylvestre were the most efficient against chymotrypsin‐like proteins. All larvae midgut extracts showed trypsin‐like proteolytic activities, and the putative trypsin inhibitors from Theobroma seeds significantly inhibited purified bovine trypsin. With respect to the influence of Theobroma trypsin inhibitors on intact insects, the inclusion of T. cacao extracts in artificial diets of velvet bean caterpillars (Anticarsia gemmatalis) and sugarcane borer (Diatraea saccharalis) produced a significant increase in the percentage of adult deformation, which is directly related to both the survival rate of the insects and oviposition.

Topics

No keywords indexed for this article. Browse by subject →

References

32

[1]

Bradford MM "Interaction of partially purified amylases from Anagasta kuehniella (Lepidoptera: Pyralidae) with amylase inhibitors from wheat" Comp Biochem Physiol (1976)

[2]

10.1016/s1096-4959(00)00325-0

[3]

10.1016/0031-9422(93)85390-d

[4]

Broadway RM. "Are insects resistant to plant proteinase inhibitors?" J Insect Physiol (1995) 10.1016/0022-1910(94)00101-l

[5]

Broadway RM "Plant proteinase inhibitors: mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua" J Insect Physiol (1986) 10.1016/0022-1910(86)90097-1

[6]

10.1016/0020-1790(89)90067-x

[7]

Dodo HW "A cocoa 21 kilodalton seed protein has trypsin inhibitory activity" Café Cacao Thé (1992)

[8]

10.1016/0003-9861(61)90145-x

[9]

10.1016/0300-9629(94)90362-x

[10]

10.1016/j.abb.2004.07.022

[11]

10.1016/0022-1910(93)90071-x

[12]

10.1016/s0022-1910(97)00040-1

[13]

King EG (1985)

[14]

10.1046/j.1365-313x.1998.00119.x

[15]

10.1016/0022-1910(90)90075-q

[16]

10.1021/jf60028a003

[17]

10.1016/0022-1910(95)00043-t

[18]

10.1023/a:1002906308304

[19]

Parra JRP (1998)

[20]

10.1603/0022-0493-93.3.892

[21]

10.1046/j.1570-7458.2001.00804.x

[22]

Richardson M "Seed storage proteins: the enzyme inhibitors" Method Plant Biochem (1991)

[23]

Ryan CA "Proteinase inhibitors in plants: genes for improving defenses against insects and pathogens" Annu Rev Phytopatol (1990) 10.1146/annurev.py.28.090190.002233

[24]

10.1007/s00606-004-0223-2

[25]

SAS Institute (1990)

[26]

10.1016/s0167-7799(97)01171-2

[27]

10.2225/vol3-issue2-fulltext-3

[28]

10.1007/bf00194274

[29]

10.4161/psb.4.11.9755

[30]

10.1007/bf00015084

[31]

10.1046/j.1365-313x.1997.12020455.x

[32]

10.1016/0308-8146(93)90236-9

Cited By

6

Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors

Thais P. Souza, Renata O. Dias · 2016

Comparative Biochemistry and Physio...

Metrics

6

Citations

32

References

Details

Published: Jul 16, 2012
Vol/Issue: 81(1)
Pages: 34-52
License: View

Authors

L

Luis Cesar Maffei Sartini Paulillo

Faculdade de Tecnologia e Ciências Laboratório de Biotecnologia e Microbiologia Salvador Bahia Brazil

A

Alexandre Magno Sebbenn

Instituto Florestal do Estado de São Paulo Estação Experimental de Tupi Piracicaba São Paulo Brazil

M

Maria Tereza Vitral de Carvalho Derbyshire

Laboratório de Melhoramento de Plantas Centro de Energia Nuclear na Agricultura Universidade de São Paulo Piracicaba São Paulo Brazil

A

Aristóteles Góes‐Neto

Laboratório de Pesquisa em Microbiologia Departamento de Ciências Biológicas Universidade Estadual de Feira de Santana Feira de Santana Bahia Brazil

M

Marco Aurélio de Paula Brotto

Muscle Biology Research Group‐MUBIG University of Missouri‐Kansas City Kansas City Missouri

A

Antonio Figueira

Laboratório de Melhoramento de Plantas Centro de Energia Nuclear na Agricultura Universidade de São Paulo Piracicaba São Paulo Brazil

Cite This Article

Luis Cesar Maffei Sartini Paulillo, Alexandre Magno Sebbenn, Maria Tereza Vitral de Carvalho Derbyshire, et al. (2012). EVALUATION OF IN VITRO AND IN VIVO EFFECTS OF SEMIPURIFIED PROTEINASE INHIBITORS FROM THEOBROMA SEEDS ON MIDGUT PROTEASE ACTIVITY OF LEPIDOPTERAN PEST INSECTS. Archives of Insect Biochemistry and Physiology, 81(1), 34-52. https://doi.org/10.1002/arch.21038

EVALUATION OF IN VITRO AND IN VIVO EFFECTS OF SEMIPURIFIED PROTEINASE INHIBITORS FROM THEOBROMA SEEDS ON MIDGUT PROTEASE ACTIVITY OF LEPIDOPTERAN PEST INSECTS

You May Also Like