Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

Xiu‐Lin Chen; Bo‐Liao Li; Yu‐Xin Chen; Guang‐Wei Li; Jun‐Xiang Wu

doi:10.1002/arch.21837

journal article Jul 22, 2021

Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

Xiu‐Lin Chen Bo‐Liao Li Yu‐Xin Chen Guang‐Wei Li Jun‐Xiang Wu

Archives of Insect Biochemistry and Physiology Vol. 108 No. 2 · Wiley

View at Publisher Save 10.1002/arch.21837

Abstract

AbstractThe olfactory system must detect and discriminate various semiochemicals in the environment. In response to such diversity, insects have evolved a family of odorant‐gated ion channels composed of a common receptor (coreceptor, Orco) and a ligand‐binding tuning odorant receptor (OR) that confers odour specificity. This study aims to examine the expression pattern of Orco gene of Grapholita molesta (GmolOrco) and to elucidate the role of GmolOrco in detecting G. molesta sex pheromone and green leaf volatiles by using gene silencing via RNA interference (RNAi) coupled antennal electrophysiological (EAG). Multiple sequence alignment showed that GmolOrco shared high sequence similarities with the Orco ortholog of lepidopterans. The results of real‐time quantitative PCR detection demonstrated that GmolOrco was predominantly expressed in adult antennae and had the highest expression quantity in adult period among the different developmental stages. Compared with the noninjected controls, GmolOrco expression in GmolOrcodouble‐stranded RNA (dsRNA)‐injected males was reduced to 39.92% and that in females was reduced to 40.43%. EAG assays showed that the responses of GmolOrco‐dsRNA injected males to sex pheromones (Z)‐8‐dodecenyl acetate (Z8‐12:OAc) and (Z)‐8‐dodecenyl alcohol (Z8‐12:OH) were significantly reduced, and the GmolOrco‐dsRNA‐injected female to green leaf volatile (Z)‐3‐hexenyl acetate also significantly declined. We inferred that Orco‐mediated olfaction was different in male and female G. molesta adults and was mainly involved in recognizing the sex pheromones released by female moths.

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Details

Published: Jul 22, 2021
Vol/Issue: 108(2)
License: View

Authors

X

Xiu‐Lin Chen

Shaanxi Province Key Laboratory of Jujube, College of Life Science Yan'an University Yan'an Shaanxi China

B

Bo‐Liao Li

Shaanxi Province Key Laboratory of Jujube, College of Life Science Yan'an University Yan'an Shaanxi China

Y

Yu‐Xin Chen

Shaanxi Province Key Laboratory of Jujube, College of Life Science Yan'an University Yan'an Shaanxi China

G

Guang‐Wei Li

Shaanxi Province Key Laboratory of Jujube, College of Life Science Yan'an University Yan'an Shaanxi China

J

Jun‐Xiang Wu

Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection Northwest A&F University Yangling Shaanxi China

Funding

National Natural Science Foundation of China Award: 31860506

Cite This Article

Xiu‐Lin Chen, Bo‐Liao Li, Yu‐Xin Chen, et al. (2021). Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae). Archives of Insect Biochemistry and Physiology, 108(2). https://doi.org/10.1002/arch.21837

Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

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