Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier

Mohammad H. Darvishi; Alireza Nomani; Hadi Hashemzadeh; Mohsen Amini; Mohammad A. Shokrgozar; Rassoul Dinarvand

doi:10.1002/bab.1497

journal article May 01, 2017

Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier

Mohammad H. Darvishi Alireza Nomani Hadi Hashemzadeh Mohsen Amini

Mohammad A. Shokrgozar Rassoul Dinarvand

Biotechnology and Applied Biochemistry Vol. 64 No. 3 pp. 423-432 · Wiley

View at Publisher Save 10.1002/bab.1497

Abstract

AbstractA novel biotinylated chitosan‐graft‐polyethyleneimine (Bio‐Chi‐g‐PEI) copolymer was synthesized and evaluated as a nonviral gene delivery carrier for improvement of the transfection efficiency, endosomal escape, and targeted gene delivery of a plasmid encoding green fluorescent protein N1 (pEGFP‐N1) into two different biotin‐overexpressing cell lines including HeLa and OVCAR‐3 cells. The structure of the obtained copolymers was confirmed by 1H nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy. Physicochemical properties of the Bio‐Chi‐g‐PEI/plasmid DNA (pDNA) complexes such as complex stability, size, zeta potential, and their morphology were investigated at various weight ratios of copolymer to pDNA. Bio‐Chi‐g‐PEI copolymers could effectively condense pDNA into small particles with average diameters less than 164 nm and the zeta potential of +34.8 mV at the N/P ratio of 40/1. As revealed by flow cytometry, Bio‐Chi‐g‐PEI/pDNA complexes had lower cytotoxicity than that of PEI 25 kDa/pDNA complexes in both cell lines. In vitro experiments revealed that the Bio‐Chi‐gPEI/pDNA complexes not only had much lower cytotoxicity, but also displayed higher transfection efficiency than that of PEI 25kDa/pDNA complexes. High percentage of cancer cells was successfully transfected by Bio‐Chi‐g‐PEI/pDNA and properly expressed GFP protein. This study indicates that this copolymer complex can be a promising gene delivery carrier.

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Details

Published: May 01, 2017
Vol/Issue: 64(3)
Pages: 423-432
License: View

Authors

M

Mohammad H. Darvishi

Nanobiotechnology Research Center Baqiyatallah University of Medical Science Tehran Iran

A

Alireza Nomani

Department of Pharmaceutics School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran

H

Hadi Hashemzadeh

Department of Nanobiotechnology Faculty of Bioscience Tarbiat Modares University Tehran Iran

M

Mohsen Amini

Department of Medicinal Chemistry Faculty of Pharmacy Tehran University of Medical Sciences Tehran Iran

M

Mohammad A. Shokrgozar

National Cell Bank of Iran (NCBI) Pasteur Institute of Iran Tehran Iran

R

Rassoul Dinarvand

Nanotechnology Research Centre Tehran University of Medical Sciences Tehran Iran; Department of Pharmaceutics Faculty of Pharmacy Tehran University of Medical Science Tehran Iran

Cite This Article

Mohammad H. Darvishi, Alireza Nomani, Hadi Hashemzadeh, et al. (2017). Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier. Biotechnology and Applied Biochemistry, 64(3), 423-432. https://doi.org/10.1002/bab.1497

Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier

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