Investigation of autophagy and differentiation of myenteric interstitial cells of Cajal in the pathogenesis of gastric motility disorders in rats with functional dyspepsia

Li‐min Zhang; Li‐jun Zeng; Jing Deng; Yu‐qin Zhang; Yu‐Jiao Wang; Tian‐yi Xie; Jiang‐hong Ling

doi:10.1002/bab.1635

journal article Feb 07, 2018

Investigation of autophagy and differentiation of myenteric interstitial cells of Cajal in the pathogenesis of gastric motility disorders in rats with functional dyspepsia

Li‐min Zhang

Li‐jun Zeng Jing Deng

Yu‐qin Zhang Yu‐Jiao Wang Tian‐yi Xie Jiang‐hong Ling

Biotechnology and Applied Biochemistry Vol. 65 No. 4 pp. 533-539 · Wiley

View at Publisher Save 10.1002/bab.1635

Abstract

AbstractInterstitial cells of Cajal (ICC), especially myenteric interstitial cells of Cajal (ICC‐MY), are key to gastrointestinal motility. However, their role in the pathogenesis of functional dyspepsia (FD) is unclear. Therefore, autophagy and differentiation of ICC‐MY were investigated to elucidate the pathogenesis of gastric motility disorder in FD. FD model was induced by chronic stress via tail clamping in rats, which was assessed by the vital signs of rats, gastric emptying rate result, and histology. The ultrastructure of ICC‐MY was examined using transmission electron microscope. In ICC‐MY, changes in autophagic biomarkers (Beclin1 and LC3B) and differentiation biomarkers (c‐kit and SCF) were evaluated with in situ hybridization, quantitative real time PCR, immunofluorescence, and Western blot, respectively. The FD model was successfully induced in rats, as evidenced by the abnormal vital signs (such as loss of appetite, liquid excreta, less activity, and slower weight gain), the decrease in gastric emptying rates, and little pathological change in gastric antrum tissue. Compared with the control group, FD caused increased organelle denaturation or reduction and increase in vacuolization. FD also promoted generation of autophagosomes in ICC‐MY. Moreover, increased the expression of Beclin1 and LC3B, but decreased expression of c‐kit and SCF. Excessive autophagy and abnormal differentiation of ICC‐MY may contribute to the pathogenesis of gastric motility disorder in FD.

Topics

No keywords indexed for this article. Browse by subject →

References

30

[1]

10.1053/gast.1996.v111.pm8690216

[2]

10.1254/jphs.crj04002x

[3]

10.1111/j.1572-0241.2002.05851.x

[4]

10.1046/j.1440-1746.2002.02756.x

[5]

10.1097/00000478-200302000-00012

[6]

Villanacci V. J. Clin. Gastroenterol (2010) 10.1097/mcg.0b013e3181bc9ebf

[7]

10.1152/ajpgi.00362.2001

[8]

10.1111/j.1582-4934.2008.00352.x

[9]

Epidemiology of functional dyspepsia: A global perspective

Sanjiv Mahadeva

World Journal of Gastroenterology 10.3748/wjg.v12.i17.2661

[10]

10.1111/j.1440-1746.2011.06633.x

[11]

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J Klionsky, Kotb Abdelmohsen, Akihisa Abe et al.

Autophagy 10.1080/15548627.2015.1100356

[12]

Dai Y. Chin. J. Integr. Tradit. West. Med. Dig (2017)

[13]

10.1016/s0016-5085(99)70560-3

[14]

10.1016/s0016-5085(09)60235-3

[15]

Ling J. China J. Chin. Mater. Med (2010)

[16]

10.11569/wcjd.v22.i2.210

[17]

10.1016/0024-3205(91)90405-z

[18]

10.1007/s00441-003-0835-9

[19]

10.3748/wjg.v11.i18.2838

[20]

10.1038/nrgastro.2013.10

[21]

10.2174/156652408784533751

[22]

10.12659/msm.899023

[23]

Methods in Mammalian Autophagy Research

Noboru Mizushima, Tamotsu Yoshimori, Beth Levine

Cell 10.1016/j.cell.2010.01.028

[24]

10.1016/j.etp.2010.11.008

[25]

10.1038/373347a0

[26]

Development of c-Kit-positive cells and the onset of electrical rhythmicity in murine small intestine

S Torihashi, SM Ward, KM Sanders

Gastroenterology 10.1016/s0016-5085(97)70229-4

[27]

10.1152/ajpgi.2001.281.3.g602

[28]

10.1016/s0024-3205(02)01517-5

[29]

The Role of Autophagy in Mammalian Development: Cell Makeover Rather than Cell Death

Francesco Cecconi, Beth Levine

Developmental Cell 10.1016/j.devcel.2008.08.012

[30]

10.1016/j.cell.2007.02.023

Metrics

32

Citations

30

References

Details

Published: Feb 07, 2018
Vol/Issue: 65(4)
Pages: 533-539
License: View

Authors

L

Li‐min Zhang