Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus

Mohammad AFZAL; Sosamma Oommen; Samira Al‐Awadi

doi:10.1002/bab.34

journal article Jul 01, 2011

Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus

Mohammad AFZAL Sosamma Oommen Samira Al‐Awadi

Biotechnology and Applied Biochemistry Vol. 58 No. 4 pp. 250-255 · Wiley

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Abstract

AbstractWe performed a series of experiments with Geobacillus stearothermophilus, a thermophile isolated from oil‐contaminated soil in the Kuwaiti desert. The organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. In the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (CDCA). Five transformed products, namely, cholic acid, methylcholate, methylchenodeoxycholate, 3α‐hydroxy‐7‐oxo‐5β‐cholanic acid, and 7α‐hydroxy‐3‐oxo‐5β‐cholanic acid, were the major transformation products catalyzed by G. stearothermophilus. Under aerobic conditions, no evidence of side chain degradation, ring cleavage, or dehydrogenation was found among the metabolites of CDCA. CDCA transformation by a thermophile is reported for the first time.

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References

26

[1]

Hofmann A. F. New Physiol. Sci. (1999)

[2]

10.1016/j.ajpath.2010.11.026

[3]

10.3998/ark.5550190.0011.901

[4]

10.1159/000226560

[5]

10.1016/0734-9750(94)90017-5

[6]

10.1016/s0723-2020(85)80005-9

[7]

10.1001/archinte.1972.03650040071007

[8]

10.1093/ajcn/27.11.1341

[9]

Kulprecha S. Appl. Environ. Microbiol. (1985) 10.1128/aem.49.2.338-344.1985

[10]

Bell G. D. Lancet (1982)

[11]

Japanese patent 60 172 296 05.09. (1985) Nisshin Shokuhin Co. (Terada M. Horie Y. Komiyama M. Minami J. Abe N. andSato M. ursodeoxycholic acid from lithocholic acid by edible basidiomycetes).

[12]

10.1007/bf00253612

[13]

Iodoi Y. Tetrahedron (1987)

[14]

10.1016/0039-128x(86)90030-9

[15]

10.1016/s0960-0760(01)00115-7

[16]

10.1042/bj1600745

[17]

Hayakawa S. Zeitachrift fur Allgemeine Mikrobiol. (1981) 10.1002/jobm.19820220505

[18]

10.1080/10242420600906330

[19]

Al‐Hassan J. M. Int. J. Tryptophan Res. (2011)

[20]

10.1152/ajpgi.00027.2007

[21]

Hirano S. Appl. Environ. Microbiol. (1981) 10.1128/aem.42.5.912-915.1981

[22]

10.1042/bj1840613

[23]

10.1016/s0723-2020(85)80005-9

[24]

10.1016/0039-128x(93)90007-a

[25]

10.1271/bbb.58.1002

[26]

10.1016/b978-1-4832-2718-4.50010-0

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Details

Published: Jul 01, 2011
Vol/Issue: 58(4)
Pages: 250-255
License: View

Authors

M

Mohammad AFZAL

Pakistan Museum of Natural History

Cite This Article

Mohammad AFZAL, Sosamma Oommen, Samira Al‐Awadi (2011). Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus. Biotechnology and Applied Biochemistry, 58(4), 250-255. https://doi.org/10.1002/bab.34

Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus

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