Enhanced Production of Astaxanthin and Zeaxanthin by
                    Paracoccus
                    Sp. LL1 Through Random Mutagenesis

Anoth Maharjan; Beom Soo Kim

doi:10.1002/bab.70019

journal article Open Access Jun 26, 2025

Enhanced Production of Astaxanthin and Zeaxanthin by Paracoccus Sp. LL1 Through Random Mutagenesis

Anoth Maharjan

Beom Soo Kim

Biotechnology and Applied Biochemistry Vol. 73 No. 1 pp. 255-265 · Wiley

View at Publisher Save 10.1002/bab.70019

Abstract

ABSTRACT

Astaxanthin, a high‐value carotenoid with potent antioxidant and anti‐inflammatory activities, is increasingly in demand in various industries. This study reports the successful enhancement of astaxanthin and zeaxanthin production in
Paracoccus
sp. LL1 through random mutagenesis using ethyl methanesulfonate (EMS). To induce genetic diversity, EMS mutagenesis was employed, followed by the selection of mutants that exhibited increased carotenoid production. The top‐performing mutants showed a significant 2.76‐fold increase in astaxanthin and a 10.14‐fold increase in zeaxanthin compared to the wild‐type strain when treated with the optimal EMS concentration of 0.5%. The effects of initial glucose concentration and inoculum size on astaxanthin and zeaxanthin production were evaluated, and production was higher when glucose was 2% and inoculum size was 10%. Our findings demonstrate the potential of
Paracoccus
sp. LL1 as a promising alternative to traditional astaxanthin‐producing organisms, such as
Haematococcus pluvialis
, offering advantages including faster growth, simpler cultivation requirements, and genetic tractability. This approach not only enhances carotenoid production but also highlights the novelty of using
Paracoccus
sp. LL1, a less‐explored strain, for high‐yield production of both astaxanthin and zeaxanthin. These results suggest that
Paracoccus
sp. LL1 could serve as an efficient platform for industrial‐scale carotenoid production through metabolic engineering and random mutagenesis, providing a viable alternative to current production systems.

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Details

Published: Jun 26, 2025
Vol/Issue: 73(1)
Pages: 255-265
License: View

Authors

A

Anoth Maharjan

Department of Chemical Engineering Chungbuk National University Cheongju Chungbuk Republic of Korea; Bio‐Evaluation Center Korea Research Institute of Bioscience and Biotechnology Cheongju Chungbuk Republic of Korea

B

Beom Soo Kim

Department of Chemical Engineering Chungbuk National University Cheongju Chungbuk Republic of Korea

Cite This Article

Anoth Maharjan, Beom Soo Kim (2025). Enhanced Production of Astaxanthin and Zeaxanthin by Paracoccus Sp. LL1 Through Random Mutagenesis. Biotechnology and Applied Biochemistry, 73(1), 255-265. https://doi.org/10.1002/bab.70019

Enhanced Production of Astaxanthin and Zeaxanthin by Paracoccus Sp. LL1 Through Random Mutagenesis

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