Metal‐Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications

Gregory K. Hodgson; Stefania Impellizzeri

doi:10.1002/bies.70133

journal article Open Access Mar 31, 2026

Metal‐Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications

Gregory K. Hodgson

Stefania Impellizzeri

BioEssays Vol. 48 No. 4 · Wiley

View at Publisher Save 10.1002/bies.70133

Abstract

ABSTRACT
Fluorescence microscopy is essential in modern cell biology but remains constrained by photobleaching, autofluorescence, and the intrinsic quantum yields of emitters. Metal‐enhanced fluorescence (MEF) is a photophysical phenomenon in which interactions between luminescent species and metal nanostructures markedly increase emission, enabling a route to brighter, high‐contrast, noninvasive bioimaging by reshaping photophysical pathways without modifying fluorophore chemistry. This review translates MEF fundamentals into an experimental playbook for biologists, distinguishing MEF mechanisms and explaining how distance, spectral overlap, and nanoparticle morphology govern whether emission is boosted or quenched. We synthesize recent live‐cell applications—using gold and silver nanoparticles—to illustrate gains in signal‐to‐noise at lower excitation power, improved photostability, and opportunities where small‐molecule dyes often suffer low quantum yield, and provide practical guidelines for pairing dyes with metal nanostructures to lower the barrier to adopting MEF in cellular imaging.

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References

85

[1]

10.1242/jcs.090027

[2]

10.1002/ar.22554

[3]

10.1021/acssensors.2c00474

[4]

Lakowicz J. R. (2006) 10.1007/978-0-387-46312-4

[5]

Mondal P. P. (2014) 10.1007/978-94-007-7545-9

[6]

Kubitscheck U. (2017) 10.1002/9783527687732

[7]

10.1042/bsr20170031

[8]

Birk U. (2017) 10.1002/9783527802074

[9]

10.3389/fchem.2021.746900

[10]

10.1038/nbt.4106

[11]

10.1038/s41592‐019‐0403‐1

[12]

10.1016/b978‐0‐12‐391856‐7.00039‐1

[13]

10.1038/srep15348

[14]

10.1038/srep30892

[15]

10.1002/bies.201700003

[16]

10.1006/abio.2001.5377

[17]

10.1007/s11468‐005‐9002‐3

[18]

10.1016/j.aca.2011.08.020

[19]

10.1039/c3cp50206f

[20]

10.1063/1.4962034

[21]

10.1021/acs.jpcc.0c09157

[22]

10.1038/nature01937

[23]

Electromagnetic fields around silver nanoparticles and dimers

Encai Hao, George C. Schatz

The Journal of Chemical Physics 10.1063/1.1629280

[24]

10.1021/la0513353

[25]

10.1039/b604038c

[26]

10.1021/cr2001349

[27]

10.1103/physrevlett.96.113002

[28]

10.1021/acs.jpcc.9b11055

[29]

10.1039/c0cp01986k

[30]

10.1023/a:1016875709579

[31]

10.1103/physrevb.24.649

[32]

10.1088/0022‐3727/41/1/013001

[33]

10.1007/s10895‐005‐2970‐z

[34]

10.1016/j.ab.2003.09.036

[35]

10.1006/abio.2001.5503

[36]

10.1016/j.ab.2003.09.039

[37]

10.1039/b802918k

[38]

10.1088/0022‐3727/36/14/203

[39]

10.1016/j.cplett.2012.11.035

[40]

10.1016/j.bios.2018.04.007

[41]

10.1039/c8nr06156d

[42]

10.3390/bios8020046

[43]

10.3390/nano11102679

[44]

10.1016/j.talanta.2023.124818

[45]

10.1002/advs.202202051

[46]

10.1002/biot.202300519

[47]

10.1016/j.sbsr.2020.100382

[48]

Fluorescence is a random process and only a few individual molecules will emit precisely at t = τ; however across a large population of molecules τ represents the average emission time.

[49]

10.1021/jz100820m

[50]

10.1021/ja910010b

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Published: Mar 31, 2026
Vol/Issue: 48(4)
License: View

Authors

G

Gregory K. Hodgson

Department of Chemistry and Biology Toronto Metropolitan University Toronto Ontario Canada

S

Stefania Impellizzeri

Department of Chemistry and Biology Toronto Metropolitan University Toronto Ontario Canada

Funding

Natural Sciences and Engineering Research Council of Canada Award: RGPIN 2018‐0416

Cite This Article

Gregory K. Hodgson, Stefania Impellizzeri (2026). Metal‐Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications. BioEssays, 48(4). https://doi.org/10.1002/bies.70133

Metal‐Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications

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