Exosome separation using microfluidic systems: size‐based, immunoaffinity‐based and dynamic methodologies

Fang Yang; Xiangzhi Liao; Yuan Tian; Guiying Li

doi:10.1002/biot.201600699

journal article Feb 06, 2017

Exosome separation using microfluidic systems: size‐based, immunoaffinity‐based and dynamic methodologies

Fang Yang

Xiangzhi Liao Yuan Tian

Guiying Li

Biotechnology Journal Vol. 12 No. 4 · Wiley

View at Publisher Save 10.1002/biot.201600699

Abstract

AbstractExosomes, nanovesicles secreted by most types of cells, exist in virtually all bodily fluids. Their rich nucleic acid and protein content make them potentially valuable biomarkers for noninvasive molecular diagnostics. They also show promise, after further development, to serve as a drug delivery system. Unfortunately, existing exosome separation technologies, such as ultracentrifugation and methods incorporating magnetic beads, are time‐consuming, laborious and separate only exosomes of low purity. Thus, a more effective separation method is highly desirable. Microfluidic platforms are ideal tools for exosome separation, since they enable fast, cost‐efficient, portable and precise processing of nanoparticles and small volumes of liquid samples. Recently, several microfluidic‐based exosome separation technologies have been studied. In this article, the advantages of the most recent technologies, as well as their limitations, challenges and potential uses in novel microfluidic exosome separation and collection applications is reviewed. This review outlines the uses of new powerful microfluidic exosome detection tools for biologists and clinicians, as well as exosome separation tools for microfluidic engineers. Current challenges of exosome separation methodologies are also described, in order to highlight areas for future research and development.

Topics

No keywords indexed for this article. Browse by subject →

References

47

[1]

Exosomes: composition, biogenesis and function

Clotilde Théry, Laurence Zitvogel, Sebastian Amigorena

Nature Reviews Immunology 10.1038/nri855

[2]

10.1186/1745-6150-8-12

[3]

Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles

Marina Colombo, Graça Raposo, Clotilde Théry

Annual Review of Cell and Developmental Biology 10.1146/annurev-cellbio-101512-122326

[4]

10.2174/1389202916666150707154719

[5]

10.1016/j.ceb.2014.05.004

[6]

10.1146/annurev-physiol-021014-071641

[7]

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells

Hadi Valadi, Karin Ekström, Apostolos Bossios et al.

Nature Cell Biology 10.1038/ncb1596

[8]

10.1186/s40169-016-0087-4

[9]

10.1111/j.1582-4934.2006.tb00405.x

[10]

10.1016/j.bbcan.2014.04.005

[11]

10.1016/j.jconrel.2015.07.030

[12]

10.2217/bmm.13.63

[13]

10.1186/1479-5876-6-37

[14]

10.1371/journal.pone.0030679

[15]

10.1038/sj.bjc.6605058

[16]

Extracellular vesicles concentration is a promising and important parameter for industrial bioprocess monitoring

Apolonija Bedina Zavec, Veronika Kralj‐Iglic, Matjaž Brinc et al.

Biotechnology Journal 10.1002/biot.201500049

[17]

10.1016/j.ymeth.2012.01.002

[18]

10.1515/hsz-2013-0141

[19]

10.1016/s0022-1759(02)00330-7

[20]

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling

Jan Van Deun, Pieter Mestdagh, Raija Sormunen et al.

Journal of Extracellular Vesicles 10.3402/jev.v3.24858

[21]

Pedersen K. W. "Isolation and characterization of exosomes using magnetic beads" BioProbes (2013)

[22]

MSC-derived exosomes: a novel tool to treat therapy-refractory graft-versus-host disease

L Kordelas, V Rebmann, A-K Ludwig et al.

Leukemia 10.1038/leu.2014.41

[23]

10.1038/srep13103

[24]

10.1292/jvms.12-0032

[25]

10.1016/j.ymeth.2015.02.019

[26]

10.7785/tcrt.2012.500275

[27]

10.1002/elps.201100039

[28]

10.1063/1.2818767

[29]

10.1063/1.3127142

[30]

10.1002/1615-9861(200204)2:4<373::aid-prot373>3.0.co;2-i

[31]

10.1073/pnas.0604663103

[32]

10.1039/c3lc41343h

[33]

Continuous Particle Separation Through Deterministic Lateral Displacement

Lotien Richard Huang, Edward C. Cox, Robert H. Austin et al.

Science 10.1126/science.1094567

[34]

10.1007/s10544-014-9891-z

[35]

Critical particle size for fractionation by deterministic lateral displacement

David W. Inglis, John A. Davis, Robert H. Austin et al.

Lab on a Chip 10.1039/b515371a

[36]

10.1038/nnano.2016.134

[37]

10.1177/2211068216651035

[38]

10.1039/b916199f

[39]

10.1039/c4lc00136b

[40]

10.1039/c5lc01117e

[41]

10.1039/c4lc00662c

[42]

10.1016/j.snb.2016.04.091

[43]

10.1021/nn506538f

[44]

10.1039/b716851a

[45]

10.1038/nbt.2886

[46]

10.1039/c5lc00036j

[47]

10.1021/ac502082b

Cited By

204

Cancer Liquid Biopsy Using Integrated Microfluidic Exosome Analysis Platforms

Guiying Li, Weiwei Tang · 2020

Biotechnology Journal

Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases

Yizhuo Wang, Meng Zhao · 2020

Cell Death & Disease

Metrics

204

Citations

47

References

Details

Published: Feb 06, 2017
Vol/Issue: 12(4)
License: View

Authors

F

Fang Yang

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences Jilin University Changchun China

X

Xiangzhi Liao

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences Jilin University Changchun China

Y

Yuan Tian

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences Jilin University Changchun China

G

Guiying Li

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences Jilin University Changchun China; National Engineering Laboratory for AIDS Vaccine, School of Life Sciences Jilin University Changchun China

Funding

National Natural Science Foundation of China Award: 31570934

Cite This Article

Fang Yang, Xiangzhi Liao, Yuan Tian, et al. (2017). Exosome separation using microfluidic systems: size‐based, immunoaffinity‐based and dynamic methodologies. Biotechnology Journal, 12(4). https://doi.org/10.1002/biot.201600699

Exosome separation using microfluidic systems: size‐based, immunoaffinity‐based and dynamic methodologies

You May Also Like