Emodin‐Induced Necroptosis in Prostate Cancer Cells via the Mitochondrial Fission HSP90/MLKL/PGAM Pathway

Xi Zhou; Farjana Yeasmin Khusbu; Yang Xie; Peng Yang

doi:10.1002/cbdv.202201130

journal article Jun 01, 2023

Emodin‐Induced Necroptosis in Prostate Cancer Cells via the Mitochondrial Fission HSP90/MLKL/PGAM Pathway

Xi Zhou

Farjana Yeasmin Khusbu Yang Xie

Peng Yang

Chemistry & Biodiversity Vol. 20 No. 6 · Wiley

View at Publisher Save 10.1002/cbdv.202201130

Abstract

AbstractCurrently, prostate cancer is one of the major malignant tumors in males. Recurrence and metastasis are the main obstacles that prevent the effective treatment of prostate cancer. In the present study, we aimed to evaluate emodin (EG) against human prostate cancer PC3 and DU145 cells. Our study showed that EG significantly decreased the cell viability of PC3 and DU145 cells and strikingly induced non‐apoptotic cell death via necroptosis that was visualized through colony formation assay, Hoechst 33258 staining, and TEM analysis. Furthermore, RNA‐sequencing and KEGG functional enrichment analysis revealed that the necroptosis‐related pathway was activated upon EG treatment in PC3 cells. mRNA and protein expression of necroptosis markers were analyzed by qPCR and immunoblotting, which implied that EG‐induced cell necroptosis via enhancing the expression of MLKL and HSP90AA1 activating PGAM pathway which is considered as a key mediator of mitochondrial fission and leading to ROS generation in PC3 and DU145 cells. Thus, our findings suggested that EG is a new small molecule agonist that induced necroptosis in prostate cancer cells via the mitochondrial fission HSP90/MLKL/PGAM pathway.

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Details

Published: Jun 01, 2023
Vol/Issue: 20(6)
License: View

Authors

X

Xi Zhou

Xiangya Hospital of Central South University Changsha 410008 P. R. China; School of Life Sciences Central South University Changsha 410013 P. R. China

F

Farjana Yeasmin Khusbu

Xiangya School of Pharmaceutical Sciences Central South University Changsha 410013 P. R. China

Y

Yang Xie

Xiangya Hospital of Central South University Changsha 410008 P. R. China

P

Peng Yang

School of Pharmaceutical Sciences Hunan University of Medicine Huaihua 418000 P. R. China

Cite This Article

Xi Zhou, Farjana Yeasmin Khusbu, Yang Xie, et al. (2023). Emodin‐Induced Necroptosis in Prostate Cancer Cells via the Mitochondrial Fission HSP90/MLKL/PGAM Pathway. Chemistry & Biodiversity, 20(6). https://doi.org/10.1002/cbdv.202201130

Emodin‐Induced Necroptosis in Prostate Cancer Cells via the Mitochondrial Fission HSP90/MLKL/PGAM Pathway

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