DNA Compaction by Divalent Cations: Structural Specificity Revealed by the Potentiality of Designed Quaternary Diammonium Salts

Anatoly A. Zinchenko; Vladimir G. Sergeyev; Kuniaki Yamabe; Shizuaki Murata; Kenichi Yoshikawa

doi:10.1002/cbic.200300797

journal article Feb 26, 2004

DNA Compaction by Divalent Cations: Structural Specificity Revealed by the Potentiality of Designed Quaternary Diammonium Salts

Anatoly A. Zinchenko Vladimir G. Sergeyev Kuniaki Yamabe Shizuaki Murata Kenichi Yoshikawa

ChemBioChem Vol. 5 No. 3 pp. 360-368 · Wiley

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Abstract

AbstractDNA interaction with quaternary diammonium dications, R(CH3)2N+(CH2)nN+(CH3)2R, having various intercharge distances, lengths, and branching, and the chemical nature of the hydrophobic substituents were investigated by fluorescent microscopy and circular dichroism (CD) spectroscopy to reveal their structural specificity for binding to DNA. The conformational behavior of DNA was found to be highly sensitive to the structure of the dications with separated charges. The distance between two ammonium groups greatly influences the compaction activity of the dications. To explain this situation, we proposed a model that demonstrates that the charge density of the dication and the geometric fit between DNA phosphates and the ammonium groups in the dications play an important role in providing efficient DNA collapse. Elongation of the alkyl substituents (R) in the diammonium salts from ethyl to hexyl did not generate any significant alterations in the compaction activities, whereas the branching of substituents caused a drastic decrease in their compaction ability. Based on the results of CD spectroscopy, it was found that the ability of the dications to provoke a DNA transition from the B‐form to A‐form was also specific: it depended on their intercharge distances and was independent of the length of alkyl substituents.

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Details

Published: Feb 26, 2004
Vol/Issue: 5(3)
Pages: 360-368
License: View

Authors

Department of Chemistry, College of General Education, Nagoya University

K

Kenichi Yoshikawa

Cite This Article

Anatoly A. Zinchenko, Vladimir G. Sergeyev, Kuniaki Yamabe, et al. (2004). DNA Compaction by Divalent Cations: Structural Specificity Revealed by the Potentiality of Designed Quaternary Diammonium Salts. ChemBioChem, 5(3), 360-368. https://doi.org/10.1002/cbic.200300797

DNA Compaction by Divalent Cations: Structural Specificity Revealed by the Potentiality of Designed Quaternary Diammonium Salts

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