Ferritinophagy is not required for colon cancer cell growth

Mazen Hasan; Sreedhar M. Reddy; Nupur K. Das

doi:10.1002/cbin.11439

journal article Aug 14, 2020

Ferritinophagy is not required for colon cancer cell growth

Mazen Hasan Sreedhar M. Reddy Nupur K. Das

Cell Biology International Vol. 44 No. 11 pp. 2307-2314 · Wiley

View at Publisher Save 10.1002/cbin.11439

Abstract

AbstractFerritinophagy is a form of selective autophagy responsible for degrading intracellular ferritin, mediated by nuclear receptor coactivator 4 (NCOA4). NCOA4 plays significant roles in systemic iron homeostasis, and its disruption leads to simultaneous anemia and susceptibility to iron overload. The importance of iron colorectal cancer pathogenesis is well studied; however, the role of ferritinophagy in colon cancer cell growth has not been assessed. Disruption of ferritinophagy via NCOA4 knockout leads to only marginal differences in growth under basal and iron‐restricted conditions. Moreover, NCOA4 played no significant role in cell death induced by 5‐fluorouracil and erastin. Western blotting analysis for ferritin and transferrin receptor 1 found a dose‐dependent effect on expression in both proteins in wild‐type and NCOA4 knockout cell lines, but further investigation revealed no difference in growth response when treated at both high and low doses. Our data demonstrate a marginal role for ferritinophagy in growth both under normal and cytotoxic conditions in colon cancer cells, as well as a possible compensatory mechanism in colon cancer cells in response to ferroptosis induction.

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References

24

[1]

10.1182/blood-2007-12-077388

[2]

NCOA4 Deficiency Impairs Systemic Iron Homeostasis

Roberto Bellelli, Giorgia Federico, Alessandro Matte’ et al.

Cell Reports 10.1016/j.celrep.2015.12.065

[3]

Bogaert J. "Molecular genetics of colorectal cancer" Annals of Gastroenterology (2014)

[4]

Regulators of Iron Homeostasis: New Players in Metabolism, Cell Death, and Disease

Alexander R. Bogdan, Masaki Miyazawa, Kazunori Hashimoto et al.

Trends in Biochemical Sciences 10.1016/j.tibs.2015.11.012

[5]

Mechanisms of ferroptosis

Jennifer Yinuo Cao, Scott J. Dixon

Experientia 10.1007/s00018-016-2194-1

[6]

10.1007/978-1-4939-2095-2_10

[7]

10.2147/cmar.s198911

[8]

10.1182/blood.v98.3.525

[9]

10.1007/978-981-13-9589-5_2

[10]

Ferroptosis is an autophagic cell death process

Minghui Gao, Prashant Monian, Qiuhui Pan et al.

Cell Research 10.1038/cr.2016.95

[11]

10.1073/pnas.1711058114

[12]

Autophagy promotes ferroptosis by degradation of ferritin

Wen Hou, Yangchun Xie, Xinxin Song et al.

Autophagy 10.1080/15548627.2016.1187366

[13]

Transferrin and transferrin receptors update

Hiroshi Kawabata

Free Radical Biology and Medicine 10.1016/j.freeradbiomed.2018.06.037

[14]

10.1016/j.freeradbiomed.2014.12.007

[15]

10.1016/j.freeradbiomed.2011.03.014

[16]

5-Fluorouracil: mechanisms of action and clinical strategies

Daniel B. Longley, D. Paul Harkin, Patrick G. Johnston

Nature Reviews Cancer 10.1038/nrc1074

[17]

10.7554/elife.10308

[18]

Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy

Joseph D. Mancias, Xiaoxu Wang, Steven P. Gygi et al.

Nature 10.1038/nature13148

[19]

Marley A. R. "Epidemiology of colorectal cancer" International Journal of Molecular Epidemiology and Genetics (2016)

[20]

10.1016/j.cmet.2008.12.012

[21]

Shen Y. "Transferrin receptor 1 in cancer: a new sight for cancer therapy" Am J Cancer Res (2018)

[22]

10.1016/j.cmet.2016.07.015

[23]

10.3390/nu5072333

[24]

10.1158/0008-5472.can-11-3836

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Citations

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References

Details

Published: Aug 14, 2020
Vol/Issue: 44(11)
Pages: 2307-2314
License: View

Authors

M

Mazen Hasan

Department of Molecular and Integrative Physiology University of Michigan Ann Arbor Michigan

S

Sreedhar M. Reddy

Department of Molecular and Integrative Physiology University of Michigan Ann Arbor Michigan

N

Nupur K. Das

Department of Molecular and Integrative Physiology University of Michigan Ann Arbor Michigan

Cite This Article

Mazen Hasan, Sreedhar M. Reddy, Nupur K. Das (2020). Ferritinophagy is not required for colon cancer cell growth. Cell Biology International, 44(11), 2307-2314. https://doi.org/10.1002/cbin.11439

Ferritinophagy is not required for colon cancer cell growth

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