Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy

David Polcari; Samuel C. Perry; Loredano Pollegioni; Matthias Geissler; Janine Mauzeroll

doi:10.1002/celc.201600766

journal article Feb 17, 2017

Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy

David Polcari Samuel C. Perry

Loredano Pollegioni

Matthias Geissler

Janine Mauzeroll

ChemElectroChem Vol. 4 No. 4 pp. 920-926 · Wiley

View at Publisher Save 10.1002/celc.201600766

Abstract

Abstractd‐Serine acts as an endogenous co‐agonist for N‐methyl‐d‐aspartate receptors at synapses, making it essential for proper brain development and function. This amino acid has also been linked to several neurodegenerative diseases such as Alzheimer's disease and dementia. Nevertheless, the primary site and mechanism of d‐serine release remains unclear. We recently demonstrated the use of an enzymatic amperometric biosensor for the in vivo quantification of endogenous d‐serine release in Xenopus laevis tadpoles. Herein, we investigate the effect of the permselective poly(meta‐phenylenediamine) electropolymerization conditions on the biosensor's response time and selectivity. Scanning electrochemical microscopy (SECM) is then used with the optimized biosensor to measure localized release of d‐serine from a model system. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of d‐serine release in other biological samples.

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References

56

[1]

Van Horn M. R. Front. Cell. Neurosci. (2013)

[2]

[3]

10.1016/j.cell.2012.06.029

[4]

10.1038/nature08673

[5]

10.1016/s2215-0366(15)00098-x

[6]

[7]

10.1016/j.neurobiolaging.2016.03.017

[8]

10.1038/tp.2015.52

[9]

10.1016/j.neuint.2015.07.005

[10]

10.1016/j.schres.2012.09.014

[11]

[12]

10.1021/ac401631k

[13]

10.1016/j.jneumeth.2010.03.022

[14]

10.1373/clinchem.2007.086702

[15]

10.1016/j.jpba.2013.10.028

[16]

[17]

10.1021/ac702230w

[18]

10.1007/s00726-012-1365-0

[19]

10.1021/ac404111u

[20]

10.1021/ac503767n

[21]

Scanning Electrochemical Microscopy: A Comprehensive Review of Experimental Parameters from 1989 to 2015

David Polcari, Philippe Dauphin-Ducharme, Janine Mauzeroll

Chemical Reviews 10.1021/acs.chemrev.6b00067

[22]

[23]

10.1073/pnas.1214809110

[24]

10.1073/pnas.97.18.9855

[25]

10.1149/2.0701512jes

[26]

10.1039/c0ee00046a

[27]

[28]

10.1016/s0013-4686(97)00158-8

[29]

10.1021/ac00041a011

[30]

10.1016/j.jelechem.2003.07.004

[31]

10.1039/c0an00068j

[32]

10.1016/s0925-4005(03)00089-3

[33]

[34]

10.1021/ac00072a013

[35]

10.1016/j.bios.2004.10.020

[36]

10.1021/ac7021184

[37]

10.1016/s0141-0229(01)00400-8

[38]

[39]

10.1016/s0021-9258(19)85181-5

[40]

10.1007/s00253-007-1282-4

[41]

10.1021/la9001972

[42]

[43]

10.1016/j.electacta.2006.05.010

[44]

10.1016/j.electacta.2008.03.076

[45]

10.1016/j.aca.2004.03.010

[46]

10.1016/j.aca.2003.10.073

[47]

10.1201/9781420005868.ch1

[48]

10.1016/0167-4838(92)90418-d

[49]

10.1016/j.foodchem.2005.09.076

[50]

10.1177/1087057106288181

Showing 50 of 56 references

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56

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Details

Published: Feb 17, 2017
Vol/Issue: 4(4)
Pages: 920-926
License: View

Authors

D

David Polcari

Department of Chemistry McGill University, 8 01 Sherbrooke Street West Montreal, QC H3 A 0B8 Canada

S

Samuel C. Perry

Department of Chemistry McGill University, 8 01 Sherbrooke Street West Montreal, QC H3 A 0B8 Canada

L

Loredano Pollegioni

Dipartimento di Biotecnologie e Scienze della Vita Università degli studi deII'Insubria via J. H. Dunant 3 21100 Varese ltaly; The Protein Factory Centro Interuniversitario di Biotecnologie Proteiche Politecnico di Milano Università degli Studi deII'Insubria Varese Italy

M

Matthias Geissler

Life Sciences Division National Research Council of Canada 75 de Mortagne Boulevard Boucherville, QC 4B 6Y4 Canada

J

Janine Mauzeroll

Department of Chemistry McGill University, 8 01 Sherbrooke Street West Montreal, QC H3 A 0B8 Canada

Funding

Natural Sciences and Engineering Research Council of Canada

McGill University

Cite This Article

David Polcari, Samuel C. Perry, Loredano Pollegioni, et al. (2017). Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy. ChemElectroChem, 4(4), 920-926. https://doi.org/10.1002/celc.201600766

Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy

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