Multiphoton Intravital Calcium Imaging

Claire E. J. Cheetham

doi:10.1002/cpcy.40

journal article Jun 26, 2018

Multiphoton Intravital Calcium Imaging

Claire E. J. Cheetham

Current Protocols in Cytometry Vol. 85 No. 1 · Wiley

View at Publisher Save 10.1002/cpcy.40

Abstract

AbstractMultiphoton intravital calcium imaging is a powerful technique that enables high‐resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2‐photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain. The protocols in this unit enable real‐time intravital imaging of intracellular calcium concentration simultaneously in hundreds of neurons, or at the resolution of single synapses, as mice respond to sensory stimuli or perform behavioral tasks. Protocols are presented for implantation of a cranial imaging window to provide optical access to the brain and for 2‐photon image acquisition. Protocols for implantation of both open skull and thinned skull windows for single or multi‐session imaging are described. © 2018 by John Wiley & Sons, Inc.

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Details

Published: Jun 26, 2018
Vol/Issue: 85(1)
License: View

Authors

C

Claire E. J. Cheetham

Department of Neurobiology, University of Pittsburgh Pittsburgh Pennsylvania

Cite This Article

Claire E. J. Cheetham (2018). Multiphoton Intravital Calcium Imaging. Current Protocols in Cytometry, 85(1). https://doi.org/10.1002/cpcy.40

Multiphoton Intravital Calcium Imaging

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