Working with Worms: Caenorhabditis elegans as a Model Organism

Philip M. Meneely; Caroline L. Dahlberg; Jacqueline K. Rose

doi:10.1002/cpet.35

journal article Open Access Sep 03, 2019

Working with Worms: Caenorhabditis elegans as a Model Organism

Philip M. Meneely Caroline L. Dahlberg Jacqueline K. Rose

Current Protocols Essential Laboratory Techniques Vol. 19 No. 1 · Wiley

View at Publisher Save 10.1002/cpet.35

Abstract

AbstractSince its introduction as a laboratory organism 50 years ago, the nematode worm Caenorhabditis elegans has become one of the most widely used and versatile models for nearly all aspects of biological and genomic research. Many experiments in C. elegans begin with the generation and analysis of mutants that affect a specific biological process, so genetic techniques are the foundation of worm research. Many different aspects of biology are being studied in C. elegans, and three different recent Nobel Prizes have recognized six researchers working with worms. In addition, C. elegans was the first multicellular organism to have its genome sequenced, so many of the standard genomic methods have also been pioneered in C. elegans. In fact, many novel techniques and ideas are initially tested in C. elegans because of its versatility as a research organism. It is also appropriate for introducing undergraduate students to research, and some of its strengths and challenges for this purpose are discussed. © 2019 The Authors.

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References

76

[1]

10.1895/wormbook.1.47.1

[2]

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

Jasper Akerboom, Tsai-Wen Chen, Trevor J. Wardill et al.

The Journal of Neuroscience 10.1523/jneurosci.2601-12.2012

[3]

10.1101/lm.041830.116

[4]

10.1534/g3.118.200778

[5]

10.1895/wormbook.1.43.1

[6]

10.1371/journal.pone.0191802

[7]

10.1093/genetics/77.1.71

[8]

10.1534/genetics.109.104976

[9]

10.1016/j.neuron.2011.02.023

[10]

Brusca R. (2016)

[11]

10.1534/g3.112.003830

[12]

Genome Sequence of the Nematode C. elegans : A Platform for Investigating Biology

Science 10.1126/science.282.5396.2012

[13]

10.1523/jneurosci.05-04-00956.1985

[14]

Green Fluorescent Protein as a Marker for Gene Expression

Martin Chalfie, Yuan Tu, Ghia Euskirchen et al.

Science 10.1126/science.8303295

[15]

10.1371/journal.pbio.0040274

[16]

10.1073/pnas.0610877104

[17]

10.1002/wdev.165

[18]

10.1038/nmeth1075

[19]

10.1038/s41586-019-1352-7

[20]

10.1534/genetics.115.176099

[21]

Corsi A. K. Wightman B. &Chalfie M.(2018). A Transparent window into biology: A primer onCaenorhabditis elegans(WormBook). Available athttp://www.wormbook.org/chapters/www_celegansintro/celegansintro.pdf.

[22]

10.1016/j.mad.2018.08.001

[23]

10.1534/genetics.115.178335

[24]

10.1016/0092-8674(86)90004-8

[25]

10.1038/emboj.2011.22

[26]

10.1016/b978-0-12-394620-1.00006-0

[27]

10.1186/jbiol97

[28]

10.1186/1471-2148-13-10

[29]

10.7554/elife.05849

[30]

10.1038/nmeth.2889

[31]

10.1523/jneurosci.0775-07.2007

[32]

10.7554/elife.17686

[33]

10.1016/j.conb.2017.01.005

[34]

10.1073/pnas.0409009101

[35]

Greenwald I.(2005). Introduction to signal transduction (WormBook). Available athttp://wormbook.org/chapters/www_introsigtrans/introsignaltransd.html. 10.1895/wormbook.1.20.1

[36]

10.1523/jneurosci.11-01-00001.1991

[37]

10.1073/pnas.94.24.13128

[38]

Hart A. C.(2006). Behavior (WormBook).http://wormbook.org/chapters/www_behavior/behavior.html. 10.1895/wormbook.1.87.1

[39]

10.1038/378082a0

[40]

Husson S. J. Costa S. W. Schmitt C. &Gottschalk S. J.(2012).Keeping track of worm trackers. Available at:http://www.wormbook.org/chapters/www_tracking/tracking.html. 10.1895/wormbook.1.156.1

[41]

10.1111/boc.201200069

[42]

10.3390/v10020085

[43]

10.1038/nature08980

[44]

10.1016/s0896-6273(00)81196-4

[45]

10.1002/cpmb.7

[46]

Kiontke K.(2006). Ecology of Caenorhabditis species (WormBook). Available athttp://wormbook.org/chapters/www_ecolCaenorhabditis/ecolCaenorhabditis.html. 10.1895/wormbook.1.37.1

[47]

10.1186/1471-2148-11-339

[48]

10.1016/j.neulet.2013.05.053

[49]

10.1016/0092-8674(93)90529-y

[50]

10.1073/pnas.93.25.14940

Showing 50 of 76 references

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Details

Published: Sep 03, 2019
Vol/Issue: 19(1)
License: View

Authors

P

Philip M. Meneely

Department of Biology Haverford College Haverford Pennsylvania

C

Caroline L. Dahlberg

Department of Biology Western Washington University Bellingham Washington

J

Jacqueline K. Rose

Behavioral Neuroscience Program, Department of Psychology Western Washington University Bellingham Washington

Cite This Article

Philip M. Meneely, Caroline L. Dahlberg, Jacqueline K. Rose (2019). Working with Worms: Caenorhabditis elegans as a Model Organism. Current Protocols Essential Laboratory Techniques, 19(1). https://doi.org/10.1002/cpet.35

Working with Worms: Caenorhabditis elegans as a Model Organism

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