Quantification of Muscle Contraction In Vitro and In Vivo Using MUSCLEMOTION Software: From Stem Cell‐Derived Cardiomyocytes to Zebrafish and Human Hearts

Berend J. van Meer; Luca Sala; Leon G. J. Tertoolen; Godfrey L. Smith; Francis L. Burton; Christine L. Mummery

doi:10.1002/cphg.67

journal article Sep 25, 2018

Quantification of Muscle Contraction In Vitro and In Vivo Using MUSCLEMOTION Software: From Stem Cell‐Derived Cardiomyocytes to Zebrafish and Human Hearts

Berend J. van Meer Luca Sala

Leon G. J. Tertoolen Godfrey L. Smith Francis L. Burton Christine L. Mummery

Current Protocols in Human Genetics Vol. 99 No. 1 · Wiley

View at Publisher Save 10.1002/cphg.67

Abstract

AbstractQuantification of contraction is essential to the study of cardiac diseases, injury, and responses to drugs. While there are many techniques to assess contractility, most rely on costly, dedicated hardware and advanced informatics, and can only be used in specific experimental models. We have developed an automated open‐source software tool (MUSCLEMOTION) for use with standard imaging equipment, to assess contractility in vitro and in vivo and quantify responses to drugs and diseases. We describe high‐speed and disturbance‐free acquisition of images from either electrically paced or non‐paced human pluripotent stem cell‐derived cardiomyocytes, isolated adult cardiomyocytes, zebrafish hearts, and human echocardiograms. Recordings are then used as input for automated batch analysis by the MUSCLEMOTION software tool configured with specific settings and parameters tailored to the recording technique. Details on accuracy, interpretation, and troubleshooting are discussed. Acquisition duration depends on the experimental setup and aim, but quantification of drug or disease responses in an in vitro muscle model can typically be completed within a few hours. © 2018 by John Wiley & Sons, Inc.

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Metrics

26

Citations

11

References

Details

Published: Sep 25, 2018
Vol/Issue: 99(1)
License: View

Authors

B

Berend J. van Meer

Department of Anatomy and Embryology, Leiden University Medical Center Leiden The Netherlands

L

Luca Sala

Department of Anatomy and Embryology, Leiden University Medical Center Leiden The Netherlands; Present address: Istituto Auxologico Italiano, IRCCS, Center for Cardiac Arrhythmias of Genetic Origin, Laboratory of Cardiovascular Genetics, Cusano Milanino, Italy

L

Leon G. J. Tertoolen

Department of Anatomy and Embryology, Leiden University Medical Center Leiden The Netherlands

G

Godfrey L. Smith

Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Science, University of Glasgow Glasgow United Kingdom; Clyde Biosciences, BioCity Scotland, Newhouse Lanarkshire United Kingdom

F

Francis L. Burton

Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Science, University of Glasgow Glasgow United Kingdom; Clyde Biosciences, BioCity Scotland, Newhouse Lanarkshire United Kingdom

C

Christine L. Mummery

Department of Anatomy and Embryology, Leiden University Medical Center Leiden The Netherlands; Department of Applied Stem Cell Technologies, University of Twente Enschede The Netherlands

Funding

National Centre for the Replacement, Refinement and Reduction of Animals in Research Award: 35911‐259146

Cite This Article

Berend J. van Meer, Luca Sala, Leon G. J. Tertoolen, et al. (2018). Quantification of Muscle Contraction In Vitro and In Vivo Using MUSCLEMOTION Software: From Stem Cell‐Derived Cardiomyocytes to Zebrafish and Human Hearts. Current Protocols in Human Genetics, 99(1). https://doi.org/10.1002/cphg.67

Quantification of Muscle Contraction In Vitro and In Vivo Using MUSCLEMOTION Software: From Stem Cell‐Derived Cardiomyocytes to Zebrafish and Human Hearts

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