Multiple cis elements and GATA factors regulate a cuticle collagen gene in Caenorhabditis elegans

Jianghua Yin; Uday Madaan; Amy Park; Neelum Aftab; Cathy Savage‐Dunn

doi:10.1002/dvg.22847

journal article Mar 01, 2015

Multiple cis elements and GATA factors regulate a cuticle collagen gene in Caenorhabditis elegans

Jianghua Yin

Uday Madaan

Amy Park Neelum Aftab Cathy Savage‐Dunn

genesis Vol. 53 No. 3-4 pp. 278-284 · Wiley

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Abstract

SummaryThe cuticle of the nematode Caenorhabditis elegans is a specialized extracellular matrix whose major component is collagen. Cuticle collagens are encoded by a large multigene family consisting of more than 150 members. Cuticle collagen genes are expressed in epidermis (hypodermis) and may be stage‐specific or cyclically expressed. We identified cuticle collagen genes as transcriptional targets of the DBL‐1 TGF‐β‐related signaling pathway. These studies prompted us to investigate the cis‐regulatory sequences required for transcription of one of the target genes, col‐41. We generated reporter constructs that reproduce stage‐ and tissue‐specific expression of fluorescent markers. We identify four conserved sequence elements that are required for transcription of reporters. Finally, we provide evidence that col‐41 expression is controlled by a sequence element containing two GATA sites and by the epidermal GATA transcription factors ELT‐1 and ELT‐3. genesis 53:278–284, 2015. © 2015 Wiley Periodicals, Inc.

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Details

Published: Mar 01, 2015
Vol/Issue: 53(3-4)
Pages: 278-284
License: View

Authors

J

Jianghua Yin

Department of Biology Queens College CUNY, Flushing New York; The Graduate Center CUNY, New York New York

U

Uday Madaan

Department of Biology Queens College CUNY, Flushing New York; The Graduate Center CUNY, New York New York

A

Amy Park

Department of Biology Queens College CUNY, Flushing New York

N

Neelum Aftab

Department of Biology Queens College CUNY, Flushing New York

C

Cathy Savage‐Dunn

Department of Biology Queens College CUNY, Flushing New York; The Graduate Center CUNY, New York New York

Funding

National Institutes of Health

Cite This Article

Jianghua Yin, Uday Madaan, Amy Park, et al. (2015). Multiple cis elements and GATA factors regulate a cuticle collagen gene in Caenorhabditis elegans. genesis, 53(3-4), 278-284. https://doi.org/10.1002/dvg.22847

Multiple cis elements and GATA factors regulate a cuticle collagen gene in Caenorhabditis elegans

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