Differential gene expression in response to fungal pathogen exposure in the aquatic invertebrate, Daphnia dentifera

Emily E. Terrill Sondag; Tara E. Stewart Merrill; Jenny Drnevich; Jessica R. Holmes; Eva K. Fischer; Carla E. Cáceres; Lynette R. Strickland

doi:10.1002/ece3.10354

journal article Open Access Jul 28, 2023

Differential gene expression in response to fungal pathogen exposure in the aquatic invertebrate, Daphnia dentifera

Emily E. Terrill Sondag

Tara E. Stewart Merrill Jenny Drnevich

Jessica R. Holmes Eva K. Fischer

Carla E. Cáceres

Lynette R. Strickland

Ecology and Evolution Vol. 13 No. 8 · Wiley

View at Publisher Save 10.1002/ece3.10354

Abstract

AbstractWhile vertebrate immune systems are appreciated for their complexity and adaptability, invertebrate immunity is often considered to be less complex. However, immune responses in many invertebrates likely involve sophisticated processes. Interactions between the crustacean host Daphnia dentifera and its fungal pathogen Metschnikowia bicuspidata provide an excellent model for exploring the mechanisms underlying crustacean immunity. To explore the genomic basis of immunity in Daphnia, we used RNA‐sequencing technology to quantify differential gene expression between individuals of a single host genotype exposed or unexposed to M. bicuspidata over 24 h. Transcriptomic analyses showed that the number of differentially expressed genes between the control (unexposed) and experimental (exposed) groups increased over time. Gene ontology enrichment analysis revealed that differentially expressed genes were enriched for immune‐related molecules and processes, such as cuticle development, prostaglandin, and defense response processes. Our findings provide a suite of immunologically relevant genes and suggest the presence of a rapidly upregulated immune response involving the cuticle in Daphnia. Studies involving gene expression responses to pathogen exposure shine a light on the processes occurring during the course of infection. By leveraging knowledge on the genetic basis for immunity, immune mechanisms can be more thoroughly understood to refine our understanding of disease spread within invertebrate populations.

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Details

Published: Jul 28, 2023
Vol/Issue: 13(8)
License: View

Authors

E

Emily E. Terrill Sondag

Department of Evolution, Ecology, and Behavior, School of Integrative Biology University of Illinois Urbana‐Champaign Urbana Illinois USA

T

Tara E. Stewart Merrill

Department of Evolution, Ecology, and Behavior, School of Integrative Biology University of Illinois Urbana‐Champaign Urbana Illinois USA; Coastal and Marine Laboratory Florida State University St. Teresa Florida USA

J

Jenny Drnevich

High Performance Computing in Biology University of Illinois Urbana‐Champaign Urbana Illinois USA

J

Jessica R. Holmes

High Performance Computing in Biology University of Illinois Urbana‐Champaign Urbana Illinois USA

E

Eva K. Fischer

Department of Evolution, Ecology, and Behavior, School of Integrative Biology University of Illinois Urbana‐Champaign Urbana Illinois USA

C

Carla E. Cáceres

Department of Evolution, Ecology, and Behavior, School of Integrative Biology University of Illinois Urbana‐Champaign Urbana Illinois USA

L

Lynette R. Strickland

Department of Biology Boston University Boston Massachusetts USA

Funding

National Science Foundation Award: DBI‐2022049

Cite This Article

Emily E. Terrill Sondag, Tara E. Stewart Merrill, Jenny Drnevich, et al. (2023). Differential gene expression in response to fungal pathogen exposure in the aquatic invertebrate, Daphnia dentifera. Ecology and Evolution, 13(8). https://doi.org/10.1002/ece3.10354

Differential gene expression in response to fungal pathogen exposure in the aquatic invertebrate, Daphnia dentifera

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