Investigating the structure, solubility, and antibacterial properties of silver‐ and copper‐doped hydroxyapatite

Katie M. Weiss; Sierra K. Kucko; Sahar Mokhtari; Timothy J. Keenan; Anthony William Wren

doi:10.1002/jbm.b.35151

journal article Sep 02, 2022

Investigating the structure, solubility, and antibacterial properties of silver‐ and copper‐doped hydroxyapatite

Katie M. Weiss Sierra K. Kucko Sahar Mokhtari Timothy J. Keenan Anthony William Wren

Journal of Biomedical Materials Research Part B: Applied Biomaterials Vol. 111 No. 2 pp. 295-313 · Wiley

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Abstract

AbstractHydroxyapatite (HA) powders were synthesized by the wet precipitation method in which two experimental compositions were synthesized (10 mol%Ag‐HAandCu‐HA) where the CaNO3content was partially substituted with AgNO3and Cu(NO3)2. X‐ray diffraction (XRD) was employed to characterize changes to theHAstructure as the dopants (Cu2+, Ag+) were incorporated into the materials structure. Energy‐dispersive X‐ray spectroscopy (EDS) determined confirmed the compositions and found that the Ca/P ratio was 1.63 for the control (HA) whileAg‐HAandCu‐HAexhibited (X + Ca)/P ratios of 1.79 and 1.65, respectively. Antibacterial efficacies were evaluated againstE.coliandS.aureus, as a function of surface area and incubation time. The more prominent antibacterial effects were observed with bothAg‐HAandCu‐HAand the materials antibacterial influence was maintained with respect to time. Ion release studies of eachHAcomposition (15, 30, and 45 days) showed thatCu‐HAreleased significantly more Cu2+(36.1 ± 5.1 mg/L) than Ag+(2.9 ± 1.2 mg/L) fromAg‐HA. Analysis of each composition incubated in simulated body fluid (SBF) exhibited surface depositions that are likely calcium phosphate (CaP). Cytocompatibility testing in MC 3T3 Osteoblasts showed slight reductions in cell viability when tested using MTT assay, however cell adhesion studies were positive for each composition.

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Details

Published: Sep 02, 2022
Vol/Issue: 111(2)
Pages: 295-313
License: View

Authors

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Katie M. Weiss