MitoQ Mitigates Radiation‐Induced Testicular Injury: Molecular Docking and Toxicity Implications

Ayman A. Ibrahim; Marwa G. El‐Gazzar; Heba M. Karam

doi:10.1002/jbt.70447

journal article Aug 01, 2025

MitoQ Mitigates Radiation‐Induced Testicular Injury: Molecular Docking and Toxicity Implications

Ayman A. Ibrahim

Marwa G. El‐Gazzar

Heba M. Karam

Journal of Biochemical and Molecular Toxicology Vol. 39 No. 8 · Wiley

View at Publisher Save 10.1002/jbt.70447

Abstract

ABSTRACT
This study assessed the possible mitigating effect of a mitochondrion‐targeted antioxidant (MitoQ) against gamma irradiation‐induced testicular damage. The rats were distributed into five groups. The first group served as a control, the second group received MitoQ (2 mg/kg/day; i.p.) for 7 days, the third group was exposed to gamma radiation (5 gray as a single dose), the fourth group received MitoQ prophylactically before irradiation, and the last group was exposed to gamma radiation and then treated with MitoQ. The rats were killed 7 days after irradiation. MitoQ replenished mitochondrial ROS, indicating its antioxidant effect. MitoQ inhibited the intrinsic apoptosis cascade via a reduction in Bax and alleviated Bcl‐2. MitoQ improved steroidogenesis, as verified by an increase in testosterone and upregulation of 3β‐HSD and 17β‐HSD expression, in addition to increasing complex I and succinate dehydrogenase activity. Likewise, sperm evaluation corroborated these findings. The acute toxicity profile of MitoQ was investigated in mice and was found to be 75 mg/kg body weight. Additionally, a molecular docking study of MitoQ showed a good fit to the active site of succinate dehydrogenase. In conclusion, this study introduces MitoQ as a new approach for the management of testicular damage triggered by gamma irradiation.

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Details

Published: Aug 01, 2025
Vol/Issue: 39(8)
License: View

Authors

A

Ayman A. Ibrahim

Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt; Department of Chemistry and Chemical Biology McMaster University Hamilton ON Canada

M

Marwa G. El‐Gazzar

Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt

H

Heba M. Karam

Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt

Cite This Article

Ayman A. Ibrahim, Marwa G. El‐Gazzar, Heba M. Karam (2025). MitoQ Mitigates Radiation‐Induced Testicular Injury: Molecular Docking and Toxicity Implications. Journal of Biochemical and Molecular Toxicology, 39(8). https://doi.org/10.1002/jbt.70447

MitoQ Mitigates Radiation‐Induced Testicular Injury: Molecular Docking and Toxicity Implications

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