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journal article Sep 09, 2004

Adenoviral down‐regulation of osteopontin inhibits human osteoclast differentiation in vitro

Cathy J. Aitken Jason M. Hodge Geoffrey C. Nicholson
Journal of Cellular Biochemistry Vol. 93 No. 5 pp. 896-903 · Wiley
View at Publisher Save 10.1002/jcb.20247
Abstract
AbstractAlthough osteopontin (OPN) is highly expressed in osteoclasts, OPN‐deficient mice have a near‐normal bone phenotype and its role in osteoclast differentiation and function remains uncertain. We used an adenoviral OPN‐antisense vector (AdOPN‐AS) to down‐regulate OPN expression in a human in vitro osteoclastogenesis model employing CFU‐GM precursors treated with RANKL and M‐CSF. Cultures infected with AdOPN‐AS showed reduced secretion of OPN compared to cultures infected with a control adenoviral vector expressing β‐galactosidase. Infection with AdOPN‐AS co‐incident with exposure to RANKL was associated with substantial (approximately 50%) inhibition of osteoclast formation with a concomitant reduction in dentine resorption. There was also a small reduction in the size of generated osteoclasts but no significant effect on the size of resorption pits/tracks nor on the amount of resorption per osteoclast. When the cultures were infected with AdOPN‐AS after 4 days exposure to RANKL only minor effects on osteoclastogenesis were seen. Our data demonstrate that early down‐regulation of OPN in vitro inhibits human osteoclastogenesis. Since mice totally lacking OPN do not have reduced osteoclast numbers our results imply the existence in vivo of an alternative molecular pathway(s). © 2004 Wiley‐Liss, Inc.
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References
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Metrics
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Citations
33
References
Details
Published
Sep 09, 2004
Vol/Issue
93(5)
Pages
896-903
License
View
Authors
C
Cathy J. Aitken
J
Jason M. Hodge
G
Geoffrey C. Nicholson
Cite This Article
Cathy J. Aitken, Jason M. Hodge, Geoffrey C. Nicholson (2004). Adenoviral down‐regulation of osteopontin inhibits human osteoclast differentiation in vitro. Journal of Cellular Biochemistry, 93(5), 896-903. https://doi.org/10.1002/jcb.20247
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