Cross‐talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation

Dolores Baksh; Genevieve M. Boland; Rocky S. Tuan

doi:10.1002/jcb.21097

journal article Jun 01, 2007

Cross‐talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation

Dolores Baksh Genevieve M. Boland

Rocky S. Tuan

Journal of Cellular Biochemistry Vol. 101 No. 5 pp. 1109-1124 · Wiley

View at Publisher Save 10.1002/jcb.21097

Abstract

AbstractWnt signaling is involved in developmental processes and in adult stem cell homeostasis. This study analyzes the role(s) of key Wnt signaling mediators in the maintenance and osteogenesis of mesenchymal stem cells (MSCs). We focus specifically on the involvement of low‐density lipoprotein‐related protein 5 (LRP5), T‐cell factor 1 (TCF1), and Frizzled (Fz) receptors, in the presence or absence of exogenous, prototypical canonical (Wnt3a), and non‐canonical (Wnt5a) Wnts. In undifferentiated MSCs, LRP5 and TCF1 mediate canonical Wnt signal transduction, leading to increased proliferation, enhanced synergistically by Wnt3a. However, LRP5 overexpression inhibits osteogenic differentiation, further suppressed by Wnt3a. Wnt5a does not affect cell proliferation but enhances osteogenesis of MSCs. Interestingly, Wnt5a inhibits Wnt3a effects on MSCs, while Wnt3a suppresses Wnt5a‐mediated enhancement of osteogenesis. Flow cytometry revealed that LRP5 expression elicits differential changes in Fz receptor profiles in undifferentiated versus osteogenic MSCs. Taken together, these results suggest that Wnt signaling crosstalk and functional antagonism with the LRP5 co‐receptor are key signaling regulators of MSC maintenance and differentiation. J. Cell. Biochem. 101:1109–1124, 2007. © 2007 Wiley‐Liss, Inc.

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Details

Published: Jun 01, 2007
Vol/Issue: 101(5)
Pages: 1109-1124
License: View

Authors

Cite This Article

Dolores Baksh, Genevieve M. Boland, Rocky S. Tuan (2007). Cross‐talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Journal of Cellular Biochemistry, 101(5), 1109-1124. https://doi.org/10.1002/jcb.21097

Cross‐talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation

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