A novel zinc finger protein Zfp637 behaves as a repressive regulator in myogenic cellular differentiation

Kai Li; Jie Zhang; Jing‐jing Ren; Qi Wang; Kai‐yong Yang; Zhu‐juan Xiong; Yong‐qiu Mao; Yan‐yu Qi; Xiao‐wei Chen; Feng Lan; Xiu‐Jie Wang; Heng‐yi Xiao; Ping Lin; Yu‐quan Wei

doi:10.1002/jcb.22546

journal article Mar 16, 2010

A novel zinc finger protein Zfp637 behaves as a repressive regulator in myogenic cellular differentiation

Kai Li

Jie Zhang

Jing‐jing Ren Qi Wang

Kai‐yong Yang Zhu‐juan Xiong Yong‐qiu Mao Yan‐yu Qi Xiao‐wei Chen Feng Lan Xiu‐Jie Wang Heng‐yi Xiao Ping Lin

Yu‐quan Wei

Journal of Cellular Biochemistry Vol. 110 No. 2 pp. 352-362 · Wiley

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Abstract

AbstractZinc finger proteins have been implicated as transcription factors in the differentiation and development of cells and tissues in higher organisms. The classical C2H2 zinc finger motif is one main type of motif of zinc finger proteins. Our previous studies have shown that Zfp637, which comprises six consecutively typical and one atypical C2H2 zinc finger motifs, is highly expressed in undifferentiated or poorly differentiated cell lines, but is moderately or slightly expressed in normal tissues. We have also demonstrated that Zfp637 can promote cell proliferation. However, its role in the regulation of cell differentiation remains unknown. We report here that endogenous Zfp637 as well as mTERT is expressed in proliferating C2C12 myoblasts and that their expression is downregulated during myogenic differentiation. Constitutive expression of Zfp637 in C2C12 myoblasts increased mTERT expression and telomerase activity, and promoted the progression of the cell cycle and cell proliferation. By contrast, endogenous repression of Zfp637 expression by RNA interference downregulated the mTERT gene and the activity of telomerase, and markedly reduced cell proliferation. Overexpression of Zfp637 also inhibited the expression of myogenic differentiation‐specific genes such as MyoD and myogenin, and prevented C2C12 myoblast differentiation. Our results suggest that Zfp637 inhibits muscle differentiation through a defect in the cell cycle exit by potentially regulating mTERT expression in C2C12 myoblasts. This may provide a new research line for studying muscle differentiation. J. Cell. Biochem. 110: 352–362, 2010. © 2010 Wiley‐Liss, Inc.

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References

52

[1]

10.1073/pnas.0609184103

[2]

10.1016/s0925-4773(00)00423-8

[3]

10.4161/cc.8.20.9856

[4]

Bestilny LJ "Selective inhibition of telomerase activity during terminal differentiation of immortal cell lines" Cancer Res (1996)

[5]

Telomere states and cell fates

Elizabeth H. Blackburn

Nature 10.1038/35040500

[6]

10.1016/s0092-8674(01)00492-5

[7]

Extension of Life-Span by Introduction of Telomerase into Normal Human Cells

Andrea G. Bodnar, Michel Ouellette, Maria Frolkis et al.

Science 10.1126/science.279.5349.349

[8]

10.1073/pnas.92.20.9082

[9]

10.1182/blood-2006-03-012013

[10]

Chadeneau C "Telomerase activity in normal and malignant murine tissues" Oncogene (1995)

[11]

10.1128/mcb.00543-06

[12]

10.1155/2009/910610

[13]

10.1146/annurev.bi.65.070196.002005

[14]

10.1074/jbc.m602753200

[15]

10.1016/s0092-8674(01)00457-3

[16]

10.1038/345458a0

[17]

10.1074/jbc.m500877200

[18]

10.1038/346866a0

[19]

10.1038/364501a0

[20]

10.4049/jimmunol.155.8.3711

[21]

Hiyama K "Differentially expressed genes throughout the cellular immortalization processes are quite different between normal human fibroblasts and endothelial cells" Int J Oncol (2005)

[22]

10.1128/mcb.16.6.2932

[23]

10.1073/pnas.0508964102

[24]

10.1074/jbc.m011181200

[25]

Specific Association of Human Telomerase Activity with Immortal Cells and Cancer

Nam W. Kim, Mieczyslaw A. Piatyszek, Karen R. Prowse et al.

Science 10.1126/science.7605428

[26]

10.1006/bbrc.1996.1054

[27]

10.1093/nar/28.3.669

[28]

10.1189/jlb.0103014

[29]

10.1016/s0006-291x(03)01033-7

[30]

10.1073/pnas.95.18.10471

[31]

10.1128/mcb.25.18.8037-8043.2005

[32]

10.1126/science.2667136

[33]

10.1111/j.1474-9728.2004.00134.x

[34]

10.1038/364532a0

[35]

10.1126/science.277.5328.955

[36]

10.1259/bjr/57867919

[37]

10.1016/j.tibtech.2004.11.009

[38]

10.1016/s1043-4682(10)80022-4

[39]

10.1038/sj.leu.2404212

[40]

10.1073/pnas.92.11.4818

[41]

10.1016/s0303-7207(01)00550-0

[42]

10.1038/sj.onc.1201933

[43]

10.1074/jbc.m409188200

[44]

10.1073/pnas.92.26.12343

[45]

Acid blobs and negative noodles

Paul B. Sigler

Nature 10.1038/333210a0

[46]

Takakura M "Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells" Cancer Res (1999)

[47]

10.1016/s0960-9822(98)70109-5

[48]

10.1242/jcs.011668

[49]

10.1002/(sici)1520-6408(1996)18:2<173::aid-dvg10>3.0.co;2-3

[50]

10.1128/mcb.01592-06

Showing 50 of 52 references

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Details

Published: Mar 16, 2010
Vol/Issue: 110(2)
Pages: 352-362
License: View

Authors

K

Kai Li

GFZ Helmholtz Centre for Geosciences

J

Jie Zhang

Chinese Academy of Sciences

National Institute of Occupational Safety and Health

Key Laboratory of Genetic Network Biology, Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China

Cite This Article

Kai Li, Jie Zhang, Jing‐jing Ren, et al. (2010). A novel zinc finger protein Zfp637 behaves as a repressive regulator in myogenic cellular differentiation. Journal of Cellular Biochemistry, 110(2), 352-362. https://doi.org/10.1002/jcb.22546

A novel zinc finger protein Zfp637 behaves as a repressive regulator in myogenic cellular differentiation

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