Runx2 is required for postnatal intervertebral disc tissue growth and development

Lifan Liao; Hua Jiang; Yunshan Fan; Ronald S. Lu; Changli Wei; Takeshi Takarada; Shisheng He; Di Chen

doi:10.1002/jcp.27410

journal article Oct 20, 2018

Runx2 is required for postnatal intervertebral disc tissue growth and development

Lifan Liao Hua Jiang

Yunshan Fan Ronald S. Lu Changli Wei Takeshi Takarada Shisheng He Di Chen

Journal of Cellular Physiology Vol. 234 No. 5 pp. 6679-6687 · Wiley

View at Publisher Save 10.1002/jcp.27410

Abstract

AbstractRunx2 plays an essential role in embryonic disc tissue development in mice. However, the role of runt‐related transcription factor 2 (Runx2) in postnatal disc tissue growth and development has not been defined. In the present studies, we generated Runx2 conditional knockout (KO) mice (Runx2Agc1ER), in which Runx2 was deleted in Aggrecan‐expressing cells in disc tissue at postnatal 2‐weeks of age. We then analyzed changes in disc tissue growth and development using histology and immunohistochemical methods in 3‐month‐old mice. We found that large vacuolated notochordal cells were accumulated in the nucleus pulposus (NP) in Runx2 KO mice. The growth plate cartilage tissue in the disc was thicker in Runx2 KO mice. We also found a significant upregulation of Indian hedgehog (Ihh) expression in the cells in NP cells and in annulus fibrosus cells of Runx2 KO mice. These results demonstrated that Runx2 may play an important role in postnatal disc tissue development through interacting with Ihh signaling.

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Metrics

23

Citations

38

References

Details

Published: Oct 20, 2018
Vol/Issue: 234(5)
Pages: 6679-6687
License: View

Authors

L

Lifan Liao

Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois; Department of Implant Dentistry Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an Jiaotong University College of Stomatlogy Xi'an Shaanxi China

H

Hua Jiang

Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois

Y

Yunshan Fan

Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois; Department of Orthopedic Surgery Shanghai Tenth People's Hospital, Tongji University Shanghai China

R

Ronald S. Lu

Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois

C

Changli Wei

Department of Internal Medicine Rush University Medical Center Chicago Illinois

T

Takeshi Takarada

Department of Regenerative Science Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan

S

Shisheng He

Department of Orthopedic Surgery Shanghai Tenth People's Hospital, Tongji University Shanghai China

D

Di Chen

Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois

Funding

National Natural Science Foundation of China Award: 81700997

National Institute of Arthritis and Musculoskeletal and Skin Diseases Award: R01AR070222

Shenzhen Science and Technology Innovation Commission Award: JCYJ20160331114205502

Cite This Article

Lifan Liao, Hua Jiang, Yunshan Fan, et al. (2018). Runx2 is required for postnatal intervertebral disc tissue growth and development. Journal of Cellular Physiology, 234(5), 6679-6687. https://doi.org/10.1002/jcp.27410

Runx2 is required for postnatal intervertebral disc tissue growth and development

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