miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6

Gang Wu; Jiaying Tan; Junping Li; Xiaoli Sun; Lei Du; Sun Tao

doi:10.1002/jcp.28291

journal article Mar 18, 2019

miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6

Gang Wu

Jiaying Tan Junping Li Xiaoli Sun

Lei Du

Sun Tao

Journal of Cellular Physiology Vol. 234 No. 9 pp. 16281-16289 · Wiley

View at Publisher Save 10.1002/jcp.28291

Abstract

AbstractDisorders mainly caused by ischemia‐reperfusion (I/R), including stroke and myocardial infarction, is linked to debilitating health conditions and death. Recent research indicates that microRNAs (miRNAs) mediate the process of ischemic pathology. This study investigated the effects of miR‐145‐5p in regulating myocardial ischemic injury. The I/R models were established in rat cardiomyocytes H9C2 and rats. Western blot analysis and quantitative polymerase chain reaction was performed to analyze protein expression. Annexin V‐FITC/PI staining was conducted to evaluate cell apoptosis. The application of miR‐145‐5p mimics and inhibitor revealed that miR‐145‐5p promoted apoptosis in cardiomyocytes. Furthermore, we found that miR‐145‐5p directly inhibited dual specificity phosphatase 6 (DUSP6) by luciferase reporter assay. The results indicated that DUSP6 was beneficial against I/R injury through inhibiting c‐Jun N‐terminal kinase pathways. In conclusion, the essential roles of miR‐145‐5p and DUSP6 in I/R provide a novel therapeutic target to develop future intervention strategies.

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References

Details

Published: Mar 18, 2019
Vol/Issue: 234(9)
Pages: 16281-16289
License: View

Authors

G

Gang Wu

Department of Critical Care Medicine Huashan Hospital, Fudan University Shanghai People's Republic of China

J

Jiaying Tan

Department of Critical Care Medicine Huashan Hospital, Fudan University Shanghai People's Republic of China

J

Junping Li

Department of Obstetrics and Gynecology Huashan Hospital North, Fudan University Shanghai People's Republic of China

X

Xiaoli Sun

Department of Cardiology Karamay Central Hospital Karamay People's Republic of China

L

Lei Du

Department of Cardiology Karamay Central Hospital Karamay People's Republic of China

S

Sun Tao

Department of Cardiology Huashan Hospital, Fudan University Shanghai People's Republic of China

Cite This Article

Gang Wu, Jiaying Tan, Junping Li, et al. (2019). miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6. Journal of Cellular Physiology, 234(9), 16281-16289. https://doi.org/10.1002/jcp.28291

miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6

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