Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio

Ramyar Azizi; Majid Ahmadi; Shahla Danaii; Sedigheh Abdollahi‐Fard; Parisa Mosapour; Shadi Eghbal‐Fard; Sanam Dolati; Amin Kamrani; Badrossadat Rahnama; Amir Mehdizadeh; Farhad Jadidi‐Niaragh; Bahman Yousefi; Mehdi Yousefi

doi:10.1002/jcp.28543

journal article Mar 28, 2019

Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio

Ramyar Azizi Majid Ahmadi

Shahla Danaii Sedigheh Abdollahi‐Fard Parisa Mosapour Shadi Eghbal‐Fard Sanam Dolati

Amin Kamrani Badrossadat Rahnama Amir Mehdizadeh Farhad Jadidi‐Niaragh Bahman Yousefi

Mehdi Yousefi

Journal of Cellular Physiology Vol. 234 No. 10 pp. 19039-19047 · Wiley

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Abstract

AbstractRecurrent pregnancy loss (RPL) is a one of the most common obstetrical complications. Since, the successful pregnancy occurs in T helper 2 (Th2)‐dominant situation and since, Th1 type immunity is related to pregnancy failure, we investigated the effects of cyclosporine on Th1 and Th2 cells in RPL women. Totally, 76 RPL patients (38 women as treated group and 38 as control group) were included in this study. Flow cytometry was utilized to analyze the frequency of Th1 and Th2 in blood samples. Also, real‐time polymerase chain reaction was carried out to assess the messenger RNA (mRNA) expression of transcription factors and enzyme‐linked immunosorbent assay was used to evaluate Th1 and Th2 related cytokines. Significant decrease in Th1 frequency (p = 0.0004), Th1/Th2 ratio (p < 0.0001), T‐bet mRNA expression (p < 0.0001), interferon‐γ (p = 0.0007), and tumor necrosis factor α (p = 0.0002) secretion level were observed in cyclosporine group. Moreover, significant increase in Th2 frequency (p < 0.0001), mRNA expression of GATA binding protein 3 (p = 0.0001), and interleukin 10 secretion level (p = 0.0027) was also evident in treated group. At the end of the investigation, 31 (81.5%) patients in cyclosporine‐treated group had successful childbirth when compared with 16 (42.1%) women in control group (p = 0.0001). Given this, cyclosporine treatment for RPL patients with elevated Th1/Th2 ratio can result in improved pregnancy outcome.

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Details

Published: Mar 28, 2019
Vol/Issue: 234(10)
Pages: 19039-19047
License: View

Authors

R

Ramyar Azizi

Stem Cell Research Center Tabriz University of Medical Sciences Tabriz Iran; Drug Applied Research Center Tabriz University of Medical Sciences Tabriz Iran

M

Majid Ahmadi

Drug Applied Research Center Tabriz University of Medical Sciences Tabriz Iran; Student's Research Committee, School of Medicine Tabriz University of Medical Sciences Tabriz Iran

S

Shahla Danaii

Gynecology Department, Eastern Azerbaijan ACECR ART Center Eastern Azerbaijan Branch of ACECR Tabriz Iran

S

Sedigheh Abdollahi‐Fard

Gynecology Department, Eastern Azerbaijan ACECR ART Center Eastern Azerbaijan Branch of ACECR Tabriz Iran

P

Parisa Mosapour

Gynecology Department, Eastern Azerbaijan ACECR ART Center Eastern Azerbaijan Branch of ACECR Tabriz Iran

S

Shadi Eghbal‐Fard

Stem Cell Research Center Tabriz University of Medical Sciences Tabriz Iran; Department of Immunology, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran

S

Sanam Dolati

Department of Immunology, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran; Aging Research Institute Tabriz University of Medical Sciences Tabriz Iran

A

Amin Kamrani

Department of Immunology, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran

B

Badrossadat Rahnama

Women's Reproductive Health Research Center Tabriz University of Medical Sciences Tabriz Iran

A

Amir Mehdizadeh

Endocrine Research Center Tabriz University of Medical Sciences Tabriz Iran

F

Farhad Jadidi‐Niaragh

Drug Applied Research Center Tabriz University of Medical Sciences Tabriz Iran; Department of Immunology, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran

B

Bahman Yousefi

Drug Applied Research Center Tabriz University of Medical Sciences Tabriz Iran

M

Mehdi Yousefi

Stem Cell Research Center Tabriz University of Medical Sciences Tabriz Iran; Drug Applied Research Center Tabriz University of Medical Sciences Tabriz Iran; Department of Immunology, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran

Funding

Tabriz University of Medical Sciences

Cite This Article

Ramyar Azizi, Majid Ahmadi, Shahla Danaii, et al. (2019). Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio. Journal of Cellular Physiology, 234(10), 19039-19047. https://doi.org/10.1002/jcp.28543

Cyclosporine A improves pregnancy outcomes in women with recurrent pregnancy loss and elevated Th1/Th2 ratio

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