Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination

Vasiliy S. Chernyshev; Roman N. Chuprov‐Netochin; Ekaterina Tsydenzhapova; Elena V. Svirshchevskaya; Rimma A. Poltavtseva; Anastasiia Merdalimova; Alexey Yashchenok; Amiran Keshelava; Konstantin Sorokin; Varlam Keshelava; Gennadiy T. Sukhikh; Dmitry Gorin; Sergey Leonov; Mikhail Skliar

doi:10.1002/jev2.12256

journal article Open Access Aug 01, 2022

Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination

Vasiliy S. Chernyshev

Roman N. Chuprov‐Netochin Ekaterina Tsydenzhapova Elena V. Svirshchevskaya

Rimma A. Poltavtseva Anastasiia Merdalimova Alexey Yashchenok Amiran Keshelava Konstantin Sorokin Varlam Keshelava Gennadiy T. Sukhikh Dmitry Gorin Sergey Leonov

Mikhail Skliar

Journal of Extracellular Vesicles Vol. 11 No. 8 · Wiley

View at Publisher Save 10.1002/jev2.12256

Abstract

Abstract
We developed a novel asymmetric depth filtration (DF) approach to isolate extracellular vesicles (EVs) from biological fluids that outperforms ultracentrifugation and size‐exclusion chromatography in purity and yield of isolated EVs. By these metrics, a single‐step DF matches or exceeds the performance of multistep protocols with dedicated purification procedures in the isolation of plasma EVs. We demonstrate the selective transit and capture of biological nanoparticles in asymmetric pores by size and elasticity, low surface binding to the filtration medium, and the ability to cleanse EVs held by the filter before their recovery with the reversed flow all contribute to the achieved purity and yield of preparations. We further demonstrate the method's versatility by applying it to isolate EVs from different biofluids (plasma, urine, and cell culture growth medium). The DF workflow is simple, fast, and inexpensive. Only standard laboratory equipment is required for its implementation, making DF suitable for low‐resource and point‐of‐use locations. The method may be used for EV isolation from small biological samples in diagnostic and treatment guidance applications. It can also be scaled up to harvest therapeutic EVs from large volumes of cell culture medium.

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Details

Published: Aug 01, 2022
Vol/Issue: 11(8)
License: View

Authors

V

Vasiliy S. Chernyshev

Skolkovo Institute of Science and Technology Moscow Russian Federation; School of Biological and Medical Physics Moscow Institute of Physics and Technology Dolgoprudny Moscow Region Russian Federation

R

Roman N. Chuprov‐Netochin

School of Biological and Medical Physics Moscow Institute of Physics and Technology Dolgoprudny Moscow Region Russian Federation

E

Ekaterina Tsydenzhapova

School of Biological and Medical Physics Moscow Institute of Physics and Technology Dolgoprudny Moscow Region Russian Federation

E

Elena V. Svirshchevskaya

Shemyakin‐Ovchinnikov Institute of Bioorganic Chemistry RAS Moscow Russian Federation

R

Rimma A. Poltavtseva

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Healthcare of the Russian Federation Moscow Russian Federation

A

Anastasiia Merdalimova

Skolkovo Institute of Science and Technology Moscow Russian Federation

A

Alexey Yashchenok

Skolkovo Institute of Science and Technology Moscow Russian Federation

A

Amiran Keshelava

Prostagnost LLC, Moscow Russian Federation

K

Konstantin Sorokin

Prostagnost LLC, Moscow Russian Federation

V

Varlam Keshelava

Institute for Biological Instrumentation RAS Pushchino Russian Federation

G

Gennadiy T. Sukhikh

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Healthcare of the Russian Federation Moscow Russian Federation

D

Dmitry Gorin

Skolkovo Institute of Science and Technology Moscow Russian Federation

S

Sergey Leonov

School of Biological and Medical Physics Moscow Institute of Physics and Technology Dolgoprudny Moscow Region Russian Federation

M

Mikhail Skliar

Department of Chemical Engineering University of Utah Salt Lake City UT USA; The Nano Institute of Utah University of Utah Salt Lake City UT USA

Cite This Article

Vasiliy S. Chernyshev, Roman N. Chuprov‐Netochin, Ekaterina Tsydenzhapova, et al. (2022). Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination. Journal of Extracellular Vesicles, 11(8). https://doi.org/10.1002/jev2.12256

Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination

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