Peripheral blood mononuclear cells enhance the anabolic effects of platelet‐rich plasma on anterior cruciate ligament fibroblasts

Ryu Yoshida; Martha M. Murray

doi:10.1002/jor.22183

journal article Jul 05, 2012

Peripheral blood mononuclear cells enhance the anabolic effects of platelet‐rich plasma on anterior cruciate ligament fibroblasts

Ryu Yoshida Martha M. Murray

Journal of Orthopaedic Research Vol. 31 No. 1 pp. 29-34 · Wiley

View at Publisher Save 10.1002/jor.22183

Abstract

AbstractUse of platelet‐rich plasma (PRP) has shown promise in various orthopaedic applications, including treatment of anterior cruciate ligament (ACL) injuries. However, various components of blood, including peripheral blood mononuclear cells (PBMCs), are removed in the process of making PRP. It is yet unknown whether these PBMCs have a positive or negative effect on fibroblast behavior. To begin to define the effect of PBMCs on ACL fibroblasts, ACL fibroblasts were cultured on three‐dimensional collagen scaffolds for 14 days with and without PBMCs. ACL fibroblasts exposed to PBMCs showed increased type I and type III procollagen gene expression, collagen protein expression, and cell proliferation when the cells were cultured in the presence of platelets and plasma. However, addition of PBMCs to cells cultured without platelets had no effect. The increase in collagen gene and protein expression was accompanied by an increase in IL‐6 expression by the PBMCs with exposure to the platelets. Our results suggest that the interaction between platelets and PBMCs leads to an IL‐6 mediated increase in collagen expression by ACL fibroblasts. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31:29–34, 2012

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Metrics

49

Citations

36

References

Details

Published: Jul 05, 2012
Vol/Issue: 31(1)
Pages: 29-34
License: View

Authors

R

Ryu Yoshida

Department of Orthopaedics, Cedars-Sinai Medical Center, Los Angeles, California

M

Martha M. Murray

Funding

NIH-NIAMS Award: R01 AR052772

Cite This Article

Ryu Yoshida, Martha M. Murray (2012). Peripheral blood mononuclear cells enhance the anabolic effects of platelet‐rich plasma on anterior cruciate ligament fibroblasts. Journal of Orthopaedic Research, 31(1), 29-34. https://doi.org/10.1002/jor.22183

Peripheral blood mononuclear cells enhance the anabolic effects of platelet‐rich plasma on anterior cruciate ligament fibroblasts

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