HigBA
                    toxin–antitoxin system of
                    Weissella cibaria
                    is involved in response to the bile salt stress

Ting Cai; Qiu‐Huan Zhao; Wen‐Liang Xiang; Lin Zhu; Yu Rao; Jie Tang

doi:10.1002/jsfa.12042

journal article Jun 15, 2022

HigBA toxin–antitoxin system of Weissella cibaria is involved in response to the bile salt stress

Ting Cai

Qiu‐Huan Zhao Wen‐Liang Xiang

Lin Zhu

Yu Rao

Jie Tang

Journal of the Science of Food and Agriculture Vol. 102 No. 14 pp. 6749-6756 · Wiley

View at Publisher Save 10.1002/jsfa.12042

Abstract

Abstract

BACKGROUND
Toxin–antitoxin (TA) systems are prevalent adaptive genetic elements in bacterial genomes, which can respond to environmental stress. While, few studies have addressed TA systems in probiotics and their roles in the adaptation to gastrointestinal transit (GIT) environments.

RESULTS

The
Weissella cibaria
018 could survive in pH 3.0–5.0 and 0.5–3.0 g L
−1
bile salt, and its HigBA system responded to the bile salt stress, but not to acid stress. The toxin protein HigB and its cognate antitoxin protein HigA had 85.1% and 100% similarity with those of
Lactobacillus plantarum
, respectively, and they formed the stable tetramer HigB–(HigA)
2
–HigB structure in
W. cibaria
018. When exposed to 1.5–3.0 g L
−1
bile salt, the transcriptions of
hig
B and
hig
A were up‐regulated with 4.39–19.29 and 5.94–30.91 folds, respectively. Meanwhile,
W. cibaria
018 gathered into a mass with 48.07% survival rate and its persister cells were found to increase 8.21% under 3.0 g L
−1
bile salt.

CONCLUSION

The HigBA TA system of
W. cibaria
018 responded to the bile salt stress, but not to acid stress, which might offer novel perspectives to understand the tolerant mechanism of probiotics to GIT environment. © 2022 Society of Chemical Industry.

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References

Details

Published: Jun 15, 2022
Vol/Issue: 102(14)
Pages: 6749-6756
License: View

Authors

T

Ting Cai

School of Food and Bioengineering Xihua University Chengdu China

Q

Qiu‐Huan Zhao

School of Food and Bioengineering Xihua University Chengdu China

W

Wen‐Liang Xiang

School of Food and Bioengineering Xihua University Chengdu China; Key Laboratory of Food Biotechnology of Sichuan Xihua University Chengdu China

L

Lin Zhu

School of Food and Bioengineering Xihua University Chengdu China

Y

Yu Rao

School of Food and Bioengineering Xihua University Chengdu China

J

Jie Tang

School of Food and Bioengineering Xihua University Chengdu China; Key Laboratory of Food Biotechnology of Sichuan Xihua University Chengdu China

Funding

National Natural Science Foundation of China Award: 31571935

Xihua University

Cite This Article

Ting Cai, Qiu‐Huan Zhao, Wen‐Liang Xiang, et al. (2022). HigBA toxin–antitoxin system of Weissella cibaria is involved in response to the bile salt stress. Journal of the Science of Food and Agriculture, 102(14), 6749-6756. https://doi.org/10.1002/jsfa.12042

HigBA toxin–antitoxin system of Weissella cibaria is involved in response to the bile salt stress

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