Neuroprotective effects of hydroponic ginseng fermented by Lactococcus lactisKC24 in oxidatively stressed SH‐SY5Y cells

Ji‐Young Park; Kee‐Tae Kim; Hyun‐Dong Paik

doi:10.1002/jsfa.12515

journal article Mar 09, 2023

Neuroprotective effects of hydroponic ginseng fermented by Lactococcus lactisKC24 in oxidatively stressed SH‐SY5Y cells

Ji‐Young Park Kee‐Tae Kim Hyun‐Dong Paik

Journal of the Science of Food and Agriculture Vol. 103 No. 9 pp. 4413-4420 · Wiley

View at Publisher Save 10.1002/jsfa.12515

Abstract

AbstractBACKGROUNDPanax ginseng Meyer, a traditional herb in Asia, contains bioactive compounds such as polyphenolic compounds, flavonoids, and ginsenosides. Furthermore, fermentation with probiotics can promote the biofunctional activities of ginseng. This study's object was to investigate the neuroprotective effect of hydroponic ginseng against hydrogen peroxide (H2O2)‐induced cytotoxicity and its effect on the fermentation time.RESULTSNonfermented hydroponic ginseng (HNF) was fermented with Lactococcus lactis KC24 at 37 °C for 12 h (H12F) or 24 h (H24F). As fermentation progressed, the content of ginsenosides Rd and F2 increased slightly. The viability of cells pretreated with H2O2‐exposed nonfermented soil‐cultivated ginseng (SNF), HNF, H12F, and H24F gradually improved. In addition, a similar cytotoxicity trend was observed for the level of lactate dehydrogenase released. Fermentation with L. lactis KC24 also enhanced the protective effect of HNF in all assays related to the neuroprotective pathway. In other words, superoxide dismutase and catalase messenger RNA (mRNA) expression levels were upregulated in H24F‐treated cells. Similarly, H24F also upregulated the mRNA and protein expression of brain‐derived neurotrophic factor to the highest observed concentration. Moreover, the Bax/Bcl‐2 ratio was the lowest after H24F pretreatment in H2O2‐induced SH‐SY5Y cells. Attenuating the cytotoxicity in H2O2‐induced SH‐SY5Y cells, H24F markedly reduced caspase‐3 and ‑9 mRNA expression and caspase‐3 activity.CONCLUSIONThese results suggest that HNF exhibited higher neuroprotection than SNF, which was enhanced after fermentation. This study demonstrates that H12F and H24F can be potential ingredients for developing healthy functional foods and pharmaceutical materials. © 2023 Society of Chemical Industry.

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Details

Published: Mar 09, 2023
Vol/Issue: 103(9)
Pages: 4413-4420
License: View

Authors

J

Ji‐Young Park

Department of Food Science and Biotechnology of Animal Resources Konkuk University Seoul Republic of Korea

K

Kee‐Tae Kim

Department of Food Science and Biotechnology of Animal Resources Konkuk University Seoul Republic of Korea

H

Hyun‐Dong Paik

Department of Food Science and Biotechnology of Animal Resources Konkuk University Seoul Republic of Korea

Cite This Article

Ji‐Young Park, Kee‐Tae Kim, Hyun‐Dong Paik (2023). Neuroprotective effects of hydroponic ginseng fermented by Lactococcus lactisKC24 in oxidatively stressed SH‐SY5Y cells. Journal of the Science of Food and Agriculture, 103(9), 4413-4420. https://doi.org/10.1002/jsfa.12515

Neuroprotective effects of hydroponic ginseng fermented by Lactococcus lactisKC24 in oxidatively stressed SH‐SY5Y cells

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