Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast

Zhao Xiaona; Liu Jianzhu

doi:10.1002/jsfa.6440

journal article Nov 06, 2013

Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast

Zhao Xiaona Liu Jianzhu

Journal of the Science of Food and Agriculture Vol. 94 No. 4 pp. 779-784 · Wiley

View at Publisher Save 10.1002/jsfa.6440

Abstract

AbstractBACKGROUNDTo select the antiviral active site of Scutellaria polysaccharide (SPS), safe concentrations of crude total Scutellaria polysaccharide (SPSt) and fractional polysaccharide SPS50, SPS60, SPS70 and SPS80 on chicken embryo fibroblast (CEF) were first compared using the MTT method. Then, SPSt, SPS50, SPS60, SPS70, and SPS80 at five concentrations within the safe concentration, together with Newcastle disease virus (NDV), were added to the cultivating system of CEF in three models: pre‐addition of polysaccharide, post‐addition of polysaccharide, and simultaneous addition of polysaccharides and NDV after mixing. The effects of SPS on the cellular infectivity of NDV (A570 value and the highest viral inhibitory rate) were compared using the MTT method.RESULTSAt appropriate concentrations, the five polysaccharides could significantly inhibit the infectivity of NDV on CEF. Among the five polysaccharide groups, the SPS80 group exhibited the highest viral inhibitory rate in the three sample‐addition modes.CONCLUSIONThis finding indicates that SPS80 possesses the best efficacy as a component of antiviral polysaccharide drug. © 2013 Society of Chemical Industry

Topics

No keywords indexed for this article. Browse by subject →

References

37

[1]

Shi SS "Bioactivities of polysaccharides" Chin Bull Life Sci (2011)

[2]

10.1016/j.intimp.2004.11.011

[3]

10.1016/j.ijbiomac.2010.02.004

[4]

10.1021/np050056c

[5]

10.1016/j.ijbiomac.2011.03.010

[6]

Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity

M. Zhang, S.W. Cui, P.C.K. Cheung et al.

Trends in Food Science & Technology 10.1016/j.tifs.2006.07.013

[7]

Lin ZB "Anti‐tumor and immunoregulatory activities of Canoderma lucidum and its possible mechanisms" Acta Pharmacol Sin (2004)

[8]

10.1016/j.carbpol.2008.04.040

[9]

10.1002/jsfa.3793

[10]

Li LD "Effects of 14 kinds Cordyceps polysaccharides on life of adult fruit fly" J Microbiol (2000)

[11]

Gao XM (2000)

[12]

Guan MY "Drug‐processing of Scutellaria baicalensis Georgi" Res Prac Chin Med (2003)

[13]

Cai YP "Analysis of the composition of crude Scutellaria polysaccharides" Chin J Mod Appl Pharm (2011)

[14]

Zhou SW "Immunopharmacological action of polysaccharides" Chin J Bio Pharm (1994)

[15]

Zhang DG "Antiviral effect of polysaccharide from Scutellaria baicalensis Georgi on porcine respiratory and reproductive syndrome virus" Lishizhen Med Mater Med Res (2005)

[16]

Liang Y "Effects of Astragalus polysaccharide on growth performance and immune function of broilers" Chin J Anim Nutr (2010)

[17]

10.1016/j.ijbiomac.2009.11.011

[18]

He Q "Study on polysaccharide extraction from Scutellaria baicalensis Georgi" Anhui Agric Sci Bull (2011)

[19]

Fang J "Isolation and purification methods of polysaccharide" Chin J Nat Med (2007)

[20]

Ren Z "Assay of polysaccharides in Astragalus capsules by sulphuric acid‐phenol method" J North Pharm (2006)

[21]

Liu Z "A simple and rapid method of MTT assay for evaluation of antiviral activity" Med J Wuhan Univ (2004)

[22]

Kong XF "Effects of ten Chinese herbal medicinal ingredients on proliferation of monolayer chick embryo fibroblast" J Nanjing Agric Univ (2003)

[23]

10.1016/j.ijbiomac.2007.10.019

[24]

Wei JL "Antivirus effects of flavonoids extracted from the core of Litchi chinensis against herpes simplex virus in vitro" Med J Wuhan Univ (2009)

[25]

Wang XY "Extraction of Scutellaria baicalensis Georgi polysaccharide and Codonopsis pilosula polysaccharide and their antiviral activity against NDV" Northeast Agric Univ (2011)

[26]

Li F "Studies on antivirus effect of Shuanghuanglian Injection" Chin Trad Herb Drugs (2002)

[27]

10.1016/j.ijbiomac.2004.03.002

[28]

Ren YH "The effects of astragalus polysaccharide, epimedium polysaccharide and epimedium flavone on cell‐infecting of NDV" J Nanjing Agri Univ (2001)

[29]

10.1016/s0065-3527(04)63004-1

[30]

Wang XB "The antivirus activity of Isatis root polysaccharide on PRRSV in vitro" Acta Agric Boreal Occid Sin (2009)

[31]

Liu QH "Studies on anti‐HBV effect in vitro of Limax polysaccharide" Chin J Exp Trad Med Form (2009)

[32]

10.1177/095632029500600601

[33]

Wang W "Anti‐Coxsackievirus B_3 activity of rhubarb tea in vitro" Acta Chin Med Pharm (2005)

[34]

10.1016/j.ijbiomac.2012.06.023

[35]

Chen Q (1993)

[36]

He YZ "Effects of rats' serum containing Guizhi decoction on cytopathy induced by virus" Chin J Exp Trad Med Form (1998)

[37]

Zhao Z "A simple and rapid method of MTT assay for evaluation of antiviral activity" J Hubei Med Univ (2004)

Metrics

4

Citations

37

References

Details

Published: Nov 06, 2013
Vol/Issue: 94(4)
Pages: 779-784
License: View

Authors

Z

Zhao Xiaona

College of Veterinary Medicine, Research Center for Animal Disease Control Engineering Shandong Province Shandong Agricultural University Tai'an 271018 People's Republic of China

L

Liu Jianzhu

College of Veterinary Medicine, Research Center for Animal Disease Control Engineering Shandong Province Shandong Agricultural University Tai'an 271018 People's Republic of China; Sino‐German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province Shandong Agricultural University Tai'an 271018 People's Republic of China

Cite This Article

Zhao Xiaona, Liu Jianzhu (2013). Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast. Journal of the Science of Food and Agriculture, 94(4), 779-784. https://doi.org/10.1002/jsfa.6440

Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast

You May Also Like