Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Elnaz Nourozi; Bahman Hosseini; Ramin Maleki; Babak Abdollahi Mandoulakani

doi:10.1002/jsfa.9921

journal article Aug 31, 2019

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Elnaz Nourozi

Bahman Hosseini

Ramin Maleki Babak Abdollahi Mandoulakani

Journal of the Science of Food and Agriculture Vol. 99 No. 14 pp. 6418-6430 · Wiley

View at Publisher Save 10.1002/jsfa.9921

Abstract

AbstractBACKGROUNDDracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects and with antiproliferative potential against various cancer cells. The extensive application of nanotechnology in hairy root cultures is a new sustainable production platform for producing these active constituents. In the present study, hairy roots derived from 4‐week‐old leaves and Agrobacterium rhizogenes strain ATCC15834 were used to investigate the impact of various concentrations of iron oxide nanoparticles (Fe NPs) in two elicitation time exposures (24 and 48 h) on growth, antioxidant enzyme activity, total phenolic and flavonoid content (TPC and TFC), and some polyphenols. Gene expression levels of phenylalanine ammonia‐lyase (pal) and rosmarinic acid synthase (ras) were also analyzed.RESULTSIron nanoparticles enhanced biomass accumulation in hairy roots. The treatment time and Fe NP dosage largely improved the activity of antioxidant enzymes, TPC and TFC. The highest RA (1194 μg g−1 FW) content (9.7‐fold), compared to controls, was detected with 24 h of exposure to 75 mg L−1 Fe NP, which was consistent with the expression of pal and ras genes under the influence of elicitation. The xanthomicrol, cirsimaritin, and isokaempferide content was increased 11.87, 3.85, and 2.27‐fold, respectively.CONCLUSIONStimulation of D. kotschyi hairy roots by Fe NPs led to a significant increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and xanthomicrol. © 2019 Society of Chemical Industry

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Details

Published: Aug 31, 2019
Vol/Issue: 99(14)
Pages: 6418-6430
License: View

Authors

E

Elnaz Nourozi

Department of Horticulture, Faculty of Agriculture Urmia University Urmia Iran

B

Bahman Hosseini

Department of Horticulture, Faculty of Agriculture Urmia University Urmia Iran

R

Ramin Maleki

Academic Center for Education, Culture and Research (ACECR), Urmia Branch Urmia University Urmia Iran

B

Babak Abdollahi Mandoulakani

Department of Plant Breeding and Biotechnology, Faculty of Agriculture Urmia University Urmia Iran

Funding

Urmia University Award: 4110

Cite This Article

Elnaz Nourozi, Bahman Hosseini, Ramin Maleki, et al. (2019). Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures. Journal of the Science of Food and Agriculture, 99(14), 6418-6430. https://doi.org/10.1002/jsfa.9921

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

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