Capillary Electrophoresis‐Frontal Analysis (CE‐FA) and Molecular Docking Studies on the Albumin‐Binding Properties of Dopamine and Serotonin

Zeynep Kalaycıoğlu; Defne Bilen

doi:10.1002/jssc.70041

journal article Dec 01, 2024

Capillary Electrophoresis‐Frontal Analysis (CE‐FA) and Molecular Docking Studies on the Albumin‐Binding Properties of Dopamine and Serotonin

Zeynep Kalaycıoğlu

Defne Bilen

Journal of Separation Science Vol. 47 No. 23 · Wiley

View at Publisher Save 10.1002/jssc.70041

Abstract

ABSTRACTDopamine and serotonin are neurotransmitters that are crucial for numerous physiological processes, including mood regulation, reward, and motor function. Dysregulation of these neurotransmitters is associated with various neuropsychiatric disorders. Albumin in plasma modulates the bioavailability of drugs and free concentrations of bioactive constituents. This study aimed to characterize the interactions of dopamine and serotonin with bovine serum albumin. Capillary electrophoresis in the frontal analysis mode was utilized as an effective method to assess dopamine–bovine serum albumin and serotonin–bovine serum albumin affinity. The free neurotransmitter plateaus were distinctly separated from the bovine serum albumin–neurotransmitter complex plateaus. Free dopamine and serotonin concentrations were determined by monitoring the heights of their respective plateaus. In contrast, the bound concentrations were calculated from the difference between the total and free plateau heights. Dopamine and serotonin were found to bind to bovine serum albumin at independent sites with binding constant values of 1.90 × 103 and 2.90 × 103 L/mol, respectively. Additionally, an in silico molecular docking approach revealed the binding sites for dopamine and serotonin near the glutamic acid‐291 and serine‐428 residues of bovine serum albumin, respectively.

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Details

Published: Dec 01, 2024
Vol/Issue: 47(23)
License: View

Authors

Z

Zeynep Kalaycıoğlu

Department of Chemistry Istanbul Technical University Maslak Istanbul Turkey

D

Defne Bilen

Department of Chemistry Istanbul Technical University Maslak Istanbul Turkey

Cite This Article

Zeynep Kalaycıoğlu, Defne Bilen (2024). Capillary Electrophoresis‐Frontal Analysis (CE‐FA) and Molecular Docking Studies on the Albumin‐Binding Properties of Dopamine and Serotonin. Journal of Separation Science, 47(23). https://doi.org/10.1002/jssc.70041

Capillary Electrophoresis‐Frontal Analysis (CE‐FA) and Molecular Docking Studies on the Albumin‐Binding Properties of Dopamine and Serotonin

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