Capillary electrophoresis–mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery

Walter Kolch; Christian Neusüß; Matthias Pelzing; Harald Mischak

doi:10.1002/mas.20051

journal article Mar 03, 2005

Capillary electrophoresis–mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery

Walter Kolch

Christian Neusüß

Matthias Pelzing Harald Mischak

Mass Spectrometry Reviews Vol. 24 No. 6 pp. 959-977 · Wiley

View at Publisher Save 10.1002/mas.20051

Abstract

AbstractProteome analysis is now emerging as key technology for deciphering biological processes and the discovery of biomarkers for diseases from tissues and body fluids. The complexity and wide dynamic range of protein expression poses a formidable challenge to both peptide separation technologies and mass spectrometry (MS). Here we review the efforts that have been undertaken to date, focussing on capillary electrophoresis coupled to mass spectrometry (CE‐MS). We discuss CE‐MS from an application point of view evaluating its merits and vices in regard to biomarker discovery and clinical applications. As examples, we present the use of CE‐MS for the determination of protein patterns in urine, serum, and other body fluids. Finally, the benefits and limitations of CE‐MS for the analysis of proteins in clinical samples are discussed against the background of alternative technologies. © 2005 Wiley Periodicals, Inc., Mass Spec Rev 24:959–977, 2005

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Metrics

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Citations

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References

Details

Published: Mar 03, 2005
Vol/Issue: 24(6)
Pages: 959-977
License: View

Authors

W

Walter Kolch

Systems Biology Ireland,; Conway Institute of Biomolecular & Biomedical Research, and; School of Medicine and Medical Science, University College Dublin, Dublin 4, Ireland; and

C

Christian Neusüß

Faculty of Chemistry Aalen University Aalen Germany

Cite This Article

Walter Kolch, Christian Neusüß, Matthias Pelzing, et al. (2005). Capillary electrophoresis–mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrometry Reviews, 24(6), 959-977. https://doi.org/10.1002/mas.20051

Capillary electrophoresis–mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery

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