Nerve growth factor and enhancement of proliferation, invasion, and tumorigenicity of pancreatic cancer cells

Zhaowen Zhu; Jörg Kleeff; Hany Kayed; Li Wang; Murray Korc; Markus W. Büchler; Helmut Friess

doi:10.1002/mc.10083

journal article Oct 29, 2002

Nerve growth factor and enhancement of proliferation, invasion, and tumorigenicity of pancreatic cancer cells

Zhaowen Zhu Jörg Kleeff

Hany Kayed Li Wang

Murray Korc Markus W. Büchler Helmut Friess

Molecular Carcinogenesis Vol. 35 No. 3 pp. 138-147 · Wiley

View at Publisher Save 10.1002/mc.10083

Abstract

AbstractNerve growth factor (NGF) exerts both stimulatory and inhibitory effects on neuronal and certain non‐neuronal tumors. In pancreatic cancer NGF is overexpressed, and this overexpression is associated with increased perineural invasion. NGF has the potential to stimulate the growth of some pancreatic cancer cell lines, and this effect is mediated by the phosphorylation of tyrosine kinase receptor A and mitogen‐activated protein kinase activation; it is dependent on the expression levels of tyrosine kinase receptor A and p75 receptors. To determine whether cancer cell–derived NGF can participate in the regulation of pancreatic cancer cell proliferation, PANC‐1 human pancreatic cancer cells were stably transfected with a full‐length human β‐NGF expression vector. In vitro and in vivo growth characteristics were analyzed by proliferation assays and invasion assays and in a nude mouse tumor model. Stable transfection of NGF in PANC‐1 cells resulted in enhanced anchorage‐dependent growth, with a decrease in doubling times of up to 50%, and in an approximately twofold increase in anchorage‐independent cell growth and cell invasion. Furthermore, stably transfected PANC‐1 cells showed enhanced tumorigenicity in nude mice. These results suggest that NGF has the capacity to act in a paracrine and/or an autocrine manner in pancreatic cancer and that it enhances cancer cell growth and invasion in vivo, thereby contributing to the aggressiveness and poor prognosis of this disease. © 2002 Wiley‐Liss, Inc.

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Metrics

89

Citations

41

References

Details

Published: Oct 29, 2002
Vol/Issue: 35(3)
Pages: 138-147
License: View

Authors

Chinese Academy of Sciences

Cite This Article

Zhaowen Zhu, Jörg Kleeff, Hany Kayed, et al. (2002). Nerve growth factor and enhancement of proliferation, invasion, and tumorigenicity of pancreatic cancer cells. Molecular Carcinogenesis, 35(3), 138-147. https://doi.org/10.1002/mc.10083

Nerve growth factor and enhancement of proliferation, invasion, and tumorigenicity of pancreatic cancer cells

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