Developmental changes in inhibitory effects of arsenic and heat shock on growth of pre‐implantation bovine embryos

AbstractAlthough sensitive to various disrupters, pre‐implantation embryos possess some cellular cytoprotective mechanisms that allow continued survival in the face of a deleterious environment. For stresses such as heat shock, embryonic resistance increases as development proceeds. Present objectives were to determine whether (1) arsenic compromises development of pre‐implantation bovine embryos, (2) developmental changes in embryonic resistance to arsenic mimic those seen for resistance to heat shock, and (3) developmental patterns in induction of apoptosis by arsenic are correlated with similar changes in resistance of embryos to inhibitory effects of arsenic on development. Bovine embryos produced by in vitro fertilization were exposed at the two‐cell stage or at day 5 after insemination (embryos ≥ 16‐cells in number) to either sodium arsenite (0, 1, 5, or 10 μM) or heat shock (exposure to 41°C for 0, 3, 4.5, 6, or 9 hr). Arsenic induced apoptosis and increased group 2 caspase activity for embryos at the ≥ 16‐cell stage, but not for embryos at the two‐cell stage. In contrast to these developmental changes in apoptosis responses, exposure to arsenic reduced cell number 24 hr after exposure for both two‐cell embryos and embryos ≥ 16‐cells. Similarly, the percentage of embryos that developed to the blastocyst stage at day 8 after fertilization was reduced by arsenic exposure at both stages of development. Heat shock, conversely, reduced development to the blastocyst stage when applied at the two‐cell stage, but not when applied to embryos ≥ 16‐cells at day 5 after insemination. In conclusion, arsenic can compromise development of bovine pre‐implantation embryos, the temporal window of sensitivity of embryos to arsenic is wider than for heat shock, and cellular cytoprotective responses that embryos acquire for thermal resistance are not sufficient to cause increased embryonic resistance to arsenic exposure. It is likely that despite common cellular pathologies caused by arsenic and heat shock, arsenic acts to reduce development in part through biochemical pathways not activated by heat shock. Moreover, the embryo does not acquire significant resistance to these perturbations within the time frame in development examined. Mol. Reprod. Dev. 63: 335–340, 2002. © 2002 Wiley‐Liss, Inc.