journal article Open Access Feb 01, 2026

Toward Super‐Resolution Reconstruction of Diffusion–Relaxation MRI Using Slice Excitation With Random Overlap ( SERO )

Magnetic Resonance in Medicine Vol. 95 No. 6 pp. 3213-3226 · Wiley
View at Publisher Save 10.1002/mrm.70282
Abstract
ABSTRACT

Purpose
Diffusion MRI probes tissue microstructure, but low SNR and limited resolution hinder detection of features and parameter estimates. We introduce slice excitation with random overlap (SERO), which enables variable repetition times (TRs) and diffusion weighting within a single shot. This acquisition supports super‐resolution reconstruction of baseline signal (), diffusivity (), diffusional variance (), and longitudinal relaxation () maps.


Methods

We implemented a diffusion‐weighted spin‐echo sequence in Pulseq that excites thick slices at random positions. Across shots, pseudo‐random overlap produces inter‐ and intra‐slice TR variation (0.15–21.9 s) with
b
‐values up to 1.4 ms/μm
2
. The ‐weighting enables through‐slice super‐resolution and allows estimation. Accuracy and precision were evaluated in numerical phantoms across variable SNR. SERO was compared with slice‐shifting super‐resolution and conventional high‐resolution imaging. Feasibility was demonstrated in healthy brain in vivo at 1.5‐mm isotropic resolution in 2:30 min.



Results

In simulations SERO improved accuracy of , , and while maintaining voxel‐wise precision comparable to direct sampling across SNRs. Regularized SERO achieved RMSE ≈ 0.5 μm
2
/ms () and ≈ 0.5 μm
4
/ms
2
() at SNR = 3, whereas direct sampling required SNR ≥ 7–10; root‐mean–variance decreased by > 50% versus an unregularized fit. In vivo, SERO yielded sharp tissue boundaries and smooth parameter maps.



Conclusion
Random slice overlap enriches encoding diversity, improving accuracy and precision of diffusion and relaxation parameters without longer scan time. SERO offers a novel path to high‐resolution microstructural imaging, especially at low SNR.
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Details
Published
Feb 01, 2026
Vol/Issue
95(6)
Pages
3213-3226
License
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Funding
Vetenskapsrådet Award: 2021‐04844
Knut och Alice Wallenbergs Stiftelse
Cancerfonden Award: 22 0592 JIA
Cite This Article
Felix Mortensen, Jakub Jurek, Jens Sjölund, et al. (2026). Toward Super‐Resolution Reconstruction of Diffusion–Relaxation MRI Using Slice Excitation With Random Overlap ( SERO ). Magnetic Resonance in Medicine, 95(6), 3213-3226. https://doi.org/10.1002/mrm.70282
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