Quality Control Strategies for Differentiation of
                    Kalanchoe
                    Species

Evelyn A. de Andrade; Isadora Machinski; Valter P. de Almeida; Sarah A. Barr; Wilmer H. Perera; Jane Manfron; Flávio L. Beltrame; R. Thomas Williamson; Wendy K. Strangman

doi:10.1002/pca.3525

journal article Open Access Mar 11, 2025

Quality Control Strategies for Differentiation of Kalanchoe Species

Evelyn A. de Andrade Isadora Machinski Valter P. de Almeida Sarah A. Barr Wilmer H. Perera

Jane Manfron Flávio L. Beltrame R. Thomas Williamson Wendy K. Strangman

Phytochemical Analysis Vol. 36 No. 8 pp. 2385-2400 · Wiley

View at Publisher Save 10.1002/pca.3525

Abstract

ABSTRACT

Kalanchoe
species products are commercially available in local markets and by internationally accessible online retailers and may exhibit quality issues because of misidentification from similar common names and anatomical similarities among related species used as feedstock. This study proposes an approach using UPLC‐MS/MS
E
and HPTLC, coupled with morphoanatomical analysis to establish chemical composition pattern data for five
Kalanchoe
species. Subsequently, the methods were validated by analyzing commercial products purported to contain 100%
Kalanchoe
extract. UPLC‐MS/MS
E
and HPTLC profiles demonstrated that quercetin and kaempferol derivatives were identified as the primary flavonoids in genuine plant extracts. Chemometric analysis showed clear differences in chemical profiles and no similarities between the
Kalanchoe
plant extracts and commercial products. Different patterns of anticlinal epidermal cell walls and midrib of the leaves and shape and arrangement of the vascular bundles in the petiole were the primary micro‐morphological differences observed. Evaluation of commercial samples revealed that products labeled as containing
Kalanchoe
did not match the pharmacobotanical analysis nor the chemical composition of the species. These methods can be considered important tools for quality control in commercial products derived from
Kalanchoe
species.

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Details

Published: Mar 11, 2025
Vol/Issue: 36(8)
Pages: 2385-2400
License: View

Authors

E

Evelyn A. de Andrade

State University of Ponta Grossa Ponta Grossa Paraná Brazil

I

Isadora Machinski

State University of Ponta Grossa Ponta Grossa Paraná Brazil

V

Valter P. de Almeida

State University of Ponta Grossa Ponta Grossa Paraná Brazil

S

Sarah A. Barr

University of North Carolina Wilmington Wilmington North Carolina USA

W

Wilmer H. Perera

CAMAG Scientific, Inc Wilmington North Carolina USA

J

Jane Manfron

State University of Ponta Grossa Ponta Grossa Paraná Brazil

F

Flávio L. Beltrame

State University of Ponta Grossa Ponta Grossa Paraná Brazil

R

R. Thomas Williamson

University of North Carolina Wilmington Wilmington North Carolina USA

W

Wendy K. Strangman

University of North Carolina Wilmington Wilmington North Carolina USA

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Award: 88881.689971/2022‐01

Cite This Article

Evelyn A. de Andrade, Isadora Machinski, Valter P. de Almeida, et al. (2025). Quality Control Strategies for Differentiation of Kalanchoe Species. Phytochemical Analysis, 36(8), 2385-2400. https://doi.org/10.1002/pca.3525

Quality Control Strategies for Differentiation of Kalanchoe Species

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