Combining selected reaction monitoring with discovery proteomics in limited biological samples

Mukesh Kumar Gupta; Jin Woo Jung; Sang Jun Uhm; Hookeun Lee; Hoon Taek Lee; Kwang Pyo Kim

doi:10.1002/pmic.200900310

journal article Oct 27, 2009

Combining selected reaction monitoring with discovery proteomics in limited biological samples

Mukesh Kumar Gupta

Jin Woo Jung Sang Jun Uhm Hookeun Lee

Hoon Taek Lee Kwang Pyo Kim

PROTEOMICS Vol. 9 No. 21 pp. 4834-4836 · Wiley

View at Publisher Save 10.1002/pmic.200900310

Abstract

AbstractSimultaneous quantification of multiple proteins by selected reaction monitoring (SRM) has several applications in cell signaling studies including embryo proteomics. However, concerns have recently been raised over the specificity of SRM assays due to possible ion redundancy and/or sequence similarity of selected peptide with multiple non‐related proteins. In this Viewpoint article, we discuss some simple measures that can increase our confidence in the accuracy of SRM scans used in proteomic experiments. At least in embryonic samples from porcine species, these measures were found to be useful in validating MS‐identified differentially expressed proteins. Among the nine proteins analyzed by SRM assay, all the proteins that were found to be up‐ or down‐regulated in MS experiment were also faithfully up‐ or down‐regulated in SRM assay.

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Metrics

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Citations

9

References

Details

Published: Oct 27, 2009
Vol/Issue: 9(21)
Pages: 4834-4836
License: View

Authors

Funding

Biogreen 21 Project of RDA Award: #200901FHT010305191, #20070401034017

Institute of Biomedical Science and Technology of Konkuk University Award: # IBST-2007-03-04

Korea Health 21 R&D Project of Ministry of Health and Welfare Award: A080611

Stem Cell Research Center for the 21st Century Frontier Research Program of Ministry of Education, Science and Technology, Republic of Korea Award: #SC-2120

Cite This Article

Mukesh Kumar Gupta, Jin Woo Jung, Sang Jun Uhm, et al. (2009). Combining selected reaction monitoring with discovery proteomics in limited biological samples. PROTEOMICS, 9(21), 4834-4836. https://doi.org/10.1002/pmic.200900310

Combining selected reaction monitoring with discovery proteomics in limited biological samples

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