Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model

Jianqiang Wu; Xundou Li; Mindi Zhao; He Huang; Wei Sun; Youhe Gao

doi:10.1002/prca.201700103

journal article Nov 20, 2017

Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model

Jianqiang Wu

Xundou Li Mindi Zhao He Huang

Wei Sun

Youhe Gao

PROTEOMICS – Clinical Applications Vol. 11 No. 11-12 · Wiley

View at Publisher Save 10.1002/prca.201700103

Abstract

ScopePulmonary fibrosis (PF) is a progressive and devastating lung disease. With limited effective treatments available in the late stage, PF has a very poor prognosis. Molecular biomarkers are highly desired for PF, especially for its early phase.Materials and methodsUrine is a good biomarker source, and accumulates systemic changes in the body especially in the early‐stage of diseases. In this study, a bleomycin (BLM)‐induced rat model is used to mimic PF. Using labeled proteome quantitation, some urinary proteins are identified as candidate biomarkers of PF for early detection and disease monitoring. Then, prednisone treatment is administered at different phases of fibrosis.ResultsOur results suggested that urine proteins could enable early detection and monitoring of both disease progression and treatment efficacy in the BLM‐induced PF model. Early prednisone treatment effectively inhibited pulmonary fibrosis, whereas the same treatment at a later phase had very limited effects. Meanwhile, five proteins showed the potential for monitoring therapeutic response.ConclusionUrinary proteomics has been underutilized in respiratory diseases. These findings will improve our understanding of the pathogenesis of PF and accelerate biomarker discovery in respiratory diseases.

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Details

Published: Nov 20, 2017
Vol/Issue: 11(11-12)
License: View

Authors

J

Jianqiang Wu

Department of Pathophysiology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing China

X

Xundou Li

Department of Pathophysiology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing China

M

Mindi Zhao

Department of Pathophysiology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing China

H

He Huang

Department of Biochemistry and Molecular Biology School of Life Sciences Beijing Normal University Gene Engineering and Biotechnology Beijing Key Laboratory Beijing China

W

Wei Sun

Core Facility of Instrument Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing China

Y

Youhe Gao

Department of Biochemistry and Molecular Biology School of Life Sciences Beijing Normal University Gene Engineering and Biotechnology Beijing Key Laboratory Beijing China

Funding

Natural Science Foundation of Beijing Municipality Award: 7173264

Beijing Normal University Award: 11100704

Cite This Article

Jianqiang Wu, Xundou Li, Mindi Zhao, et al. (2017). Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model. PROTEOMICS – Clinical Applications, 11(11-12). https://doi.org/10.1002/prca.201700103

Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model

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