journal article Open Access Mar 24, 2026

SegDesign : A modular framework for controllable protein segment engineering

Protein Science Vol. 35 No. 4 · Wiley
View at Publisher Save 10.1002/pro.70542
Abstract
Abstract

Local protein structure engineering, such as targeted remodeling of loops or flexible regions, is critical for mechanistic studies and protein optimization but remains challenging to perform in a controllable and reproducible manner. Despite advances in protein foundation models and generative structure design, most existing methods emphasize global scaffold generation and offer limited support for precise, region‐specific intervention while preserving the overall fold. Here we present SegDesign, a modular framework for segment‐level protein engineering that integrates backbone reconstruction, sequence redesign, and multi‐stage structural evaluation into a unified workflow. SegDesign enables user‐defined secondary‐structure manipulation of selected regions and produces traceable, experimentally testable variant panels. Applied to
Gallus gallus
terminal deoxynucleotidyl transferase (
Gg
TdT), SegDesign converts an intrinsically flexible loop into either a stable α‐helix or β‐strand without disrupting the global fold, as supported by AlphaFold3 modeling. Applications to six additional enzymes further demonstrate the generality of the approach. SegDesign establishes an accessible paradigm for controllable local protein structure engineering in both mechanistic and applied settings. The standalone implementation of SegDesign is publicly available at
https://github.com/mike114b/Segdesign
.
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Citations
42
References
Details
Published
Mar 24, 2026
Vol/Issue
35(4)
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Authors
Funding
Natural Science Foundation of Ningxia Province Award: 2023AAC05036
Cite This Article
Chenjie Feng, Junbo Yin, Chao Zha, et al. (2026). SegDesign : A modular framework for controllable protein segment engineering. Protein Science, 35(4). https://doi.org/10.1002/pro.70542
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