Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Debora Boaventura; Anderson Bolzan; Fernando EO Padovez; Daniela M Okuma; Celso Omoto; R. Nauen

doi:10.1002/ps.5505

journal article Jul 08, 2019

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Debora Boaventura Anderson Bolzan Fernando EO Padovez Daniela M Okuma Celso Omoto

R. Nauen

Pest Management Science Vol. 76 No. 1 pp. 47-54 · Wiley

View at Publisher Save 10.1002/ps.5505

Abstract

AbstractBACKGROUNDFall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), a major lepidopteran pest in Latin and North America, has very recently invaded the continents of Africa and Asia. FAW has evolved resistance to different insecticides and transgenic corn expressing Bacillus thuringiensis (Bt) toxins. Here, we investigated the extent and mechanisms of resistance to diamide insecticides in a Brazilian field‐collected FAW strain selected using chlorantraniliprole.RESULTSContinuous laboratory selection of a field‐collected FAW strain with chlorantraniliprole resulted in resistance ratios of 225‐fold and > 5400‐fold against chlorantraniliprole and flubendiamide, respectively, when compared with a susceptible strain. Pre‐exposure to different synergists known to inhibit detoxification enzymes did not result in significantly increased larval toxicity, suggesting a minor role for metabolic resistance. Sequencing of the FAW ryanodine receptor (RyR) C‐terminal domains II to VI revealed a single nucleotide polymorphism, resulting in a I4734M mutation recently said to confer target‐site resistance to diamides in lepidopteran pests. Genotyping by pyrosequencing of field‐collected FAW larvae sampled in the 2018 crop season suggests a low resistance allele frequency. Furthermore, we developed a fluorescent polymerase chain reaction (PCR)‐based allelic discrimination assay for rapid genotyping of field‐collected FAW samples, because diamides are increasingly used in Bt−/non‐Bt corn.CONCLUSIONSRecently, the identified RyR mutation has been shown to confer field resistance in other lepidopteran pests such as diamondback moth, tomato leafminer and striped rice stem borer. The developed PCR‐based allelic discrimination assay will help to monitor the frequency and future spread of diamide resistance allele in FAW field populations and help to implement appropriate resistance management measures. © 2019 Society of Chemical Industry

Topics

No keywords indexed for this article. Browse by subject →

References

50

[1]

A Review of the Biology of the Fall Armyworm

Alton N. Sparks

Florida Entomologist 10.2307/3494083

[2]

10.2307/3494982

[3]

First Report of Outbreaks of the Fall Armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a New Alien Invasive Pest in West and Central Africa

Georg Goergen, P. Lava Kumar, Sagnia B. Sankung et al.

PLoS ONE 10.1371/journal.pone.0165632

[4]

Studies on new invasive pest Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) and its natural enemies

A. N. SHYLESHA, S. K. JALALI, Ankita Gupta et al.

Journal of Biological Control 10.18311/jbc/2018/21707

[5]

Statista Area Planted with Corn in Brazil from Crop Year2010. Available:https://www.statista.com/statistics/740021/area‐planted‐corn‐brazil[11 April 2019].

[6]

10.1016/j.jip.2012.04.007

[7]

10.1016/j.rbe.2015.12.001

[8]

10.1371/journal.pone.0062268

[9]

10.1002/ps.4660

[10]

10.1016/j.cropro.2014.06.019

[11]

10.1093/jee/tox146

[12]

10.1603/ec14190

[13]

10.1016/j.cois.2016.04.012

[14]

10.1603/ec10040

[15]

10.1093/jee/toy372

[16]

Morillo F "Resistance of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) to lambdacyhalothrin and methomyl" Entomotropica (2001)

[17]

10.1590/s1519-566x2001000200016

[18]

10.1002/ps.4057

[19]

10.1002/ps.4829

[20]

10.1002/ps.1254

[21]

10.1002/ps.3406

[22]

10.1016/j.pestbp.2014.11.014

[23]

10.1016/j.ceca.2005.09.002

[24]

10.1016/j.pestbp.2005.07.005

[25]

Lümmen P (2013)

[26]

10.1016/j.gene.2018.05.075

[27]

10.1038/nature14063

[28]

10.1016/j.pestbp.2013.09.004

[29]

10.1021/jf501236h

[30]

10.1021/bi900866s

[31]

10.1016/j.ibmb.2013.06.006

[32]

10.1016/j.ibmb.2012.09.001

[33]

10.1603/ec12059

[34]

10.1007/s10340-015-0643-5

[35]

10.1002/ps.4439

[36]

Cho SR "Susceptibility of field populations of Plutella xylostella and Spodoptera exigua to four diamide insecticides" Korean J Appl Entomol (2018)

[37]

10.1007/978-3-319-31800-4_12

[38]

10.1016/j.ibmb.2015.05.001

[39]

10.1038/srep06924

[40]

10.1016/j.ibmb.2016.11.003

[41]

10.1038/srep14680

[42]

10.1016/j.ibmb.2017.06.013

[43]

10.1016/j.ibmb.2017.09.005

[44]

10.1093/jee/toz001

[45]

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Anderson Bolzan, Fernando EO Padovez, Antonio RB Nascimento et al.

Pest Management Science 10.1002/ps.5376

[46]

10.1002/ps.5347

[47]

FinneyDJ ProbitAnalysis.Cambridge University Press Cambridge UK p.333(1971).

[48]

10.1002/ps.4201

[49]

10.1016/j.pestbp.2013.01.010

[50]

Silva RibeiroR Monitoramento da suscetibilidade de populações deSpodoptera frugiperda(J.E. Smith) (Lepidoptera: Noctuidae) a inseticidas diamidas no Brasil. MSc dissertation Universidade de São Paulo Escola Superior de Agricultura “Luiz de Queiroz” (2014).

Cited By

170

Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance

Lianyun Lin, Changshi Wang · 2024

Nature Communications

Susceptibility monitoring and comparative gene expression of susceptible and resistant strains of Spodoptera frugiperda to lambda‐cyhalothrin and chlorpyrifos

Antonio Rogério Bezerra do Nascimento, Juliana Gonzales Rodrigues · 2023

Pest Management Science

Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP

Juil Kim, Hwa Y Nam · 2021

Pest Management Science

Metrics

170

Citations

50

References

Details

Published: Jul 08, 2019
Vol/Issue: 76(1)
Pages: 47-54
License: View

Authors

D

Debora Boaventura

Department of Molecular Phytomedicine, Institute of Crop Sciences and Resource Conservation University of Bonn Bonn Germany; Bayer AG, Crop Science Division R&D Pest Control Monheim Germany

A

Anderson Bolzan

Department of Entomology and Acarology University of São Paulo Piracicaba São Paulo Brazil

F

Fernando EO Padovez

Department of Entomology and Acarology University of São Paulo Piracicaba São Paulo Brazil

D

Daniela M Okuma

Bayer S.A., Crop Science Division, CEAT Paulinia São Paulo Brazil

C

Celso Omoto

Department of Entomology and Acarology University of São Paulo Piracicaba São Paulo Brazil

R

R. Nauen

Bayer AG, Crop Science Division R&D Pest Control Monheim Germany

Cite This Article

Debora Boaventura, Anderson Bolzan, Fernando EO Padovez, et al. (2019). Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda. Pest Management Science, 76(1), 47-54. https://doi.org/10.1002/ps.5505

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

You May Also Like