NPC1b as a novel target in controlling the cotton bollworm, Helicoverpa armigera

Jin‐Cheng Zheng; Xiao‐Rong Yue; Wen‐Qing Kuang; Sa‐Li Li; Rui Tang; Zhan‐Feng Zhang; Anwar Kurban; Saif‐Ur‐Rehman; Chaoyang Zhao; Tong‐Xian Liu; Xiangfeng Jing

doi:10.1002/ps.5761

journal article Mar 03, 2020

NPC1b as a novel target in controlling the cotton bollworm, Helicoverpa armigera

Jin‐Cheng Zheng Xiao‐Rong Yue Wen‐Qing Kuang Sa‐Li Li Rui Tang

Zhan‐Feng Zhang Anwar Kurban Saif‐Ur‐Rehman Chaoyang Zhao Tong‐Xian Liu

Xiangfeng Jing

Pest Management Science Vol. 76 No. 6 pp. 2233-2242 · Wiley

View at Publisher Save 10.1002/ps.5761

Abstract

AbstractBACKGROUNDInsects cannot synthesize sterols and must acquire them from food. The mechanisms underlying how insects uptake dietary sterols are largely unknown except that NPC1b, an integral membrane protein, has been shown to be responsible for dietary cholesterol uptake in Drosophila melanogaster. However, whether NPC1b orthologs in other insect species, particularly the economically important pests, function similarly remains to be determined.RESULTSIn this study, we characterized the function of NPC1b in Helicoverpa armigera, a global pest that causes severe yield losses to many important crops. Limiting dietary cholesterol uptake to insects significantly inhibited food ingestion and weight gain. Compared to the wild‐type H. armigera, the CRISPR/Cas9‐edited NPC1b mutant larvae were incapable of getting adequate cholesterol and died in their early life stage. Gene expression profile and in situ hybridization analyses indicated that NPC1b was mainly expressed in the midgut where dietary cholesterol was absorbed. Expression of NPC1b was also correlated with the feeding life stages and was especially upregulated during early larval instars. Protein‐ligand docking and sequence similarity analyses further demonstrated that NPC1b proteins of lepidopteran insects shared a relatively conserved cholesterol binding region, NPC1b_NTD, which, however, was highly divergent from bees‐derived sequences.CONCLUSIONNPC1b was crucial for dietary cholesterol uptake and growth of H. armigera, and therefore could serve as an insecticide target for the development of a novel pest‐management approach to control this economically significant insect pest with little off‐target effect on bees and sterol‐autotrophic animals. © 2020 Society of Chemical Industry

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Details

Published: Mar 03, 2020
Vol/Issue: 76(6)
Pages: 2233-2242
License: View

Authors

J

Jin‐Cheng Zheng

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China; Basic Medical School Guizhou University of Traditional Chinese Medicine Guiyang Guizhou China

X

Xiao‐Rong Yue

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

W

Wen‐Qing Kuang

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

S

Sa‐Li Li

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

R

Rui Tang

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

Z

Zhan‐Feng Zhang

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

A

Anwar Kurban

College of Agronomy Xinjiang Agricultural University Urumqi Xinjiang China

S

Saif‐Ur‐Rehman

College of Plant Protection Northwest A&F University Yangling Shaanxi China

C

Chaoyang Zhao

College of Natural and Agricultural Sciences University of California Riverside CA USA

T

Tong‐Xian Liu

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

X

Xiangfeng Jing

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&F University Yangling Shaanxi China; Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection, Northwest A&F University Yangling Shaanxi China

Funding

National Natural Science Foundation of China Award: 31672369

Cite This Article

Jin‐Cheng Zheng, Xiao‐Rong Yue, Wen‐Qing Kuang, et al. (2020). NPC1b as a novel target in controlling the cotton bollworm, Helicoverpa armigera. Pest Management Science, 76(6), 2233-2242. https://doi.org/10.1002/ps.5761

NPC1b as a novel target in controlling the cotton bollworm, Helicoverpa armigera

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